Blastocystis infection is associated with irritable bowel syndrome in a Mexican patient population
Por:
Jimenez-Gonzalez D.E., Martinez-Flores W.A., Reyes-Gordillo J., Ramirez-Miranda M.E., Sara Arroyo-Escalante, Romero-Valdovinos M., Stark D., Souza-Saldivar V., Martinez-Hernandez F., Flisser A., Olivo-Diaz A., Maravilla P.
Publicada:
1 mar 2012
Resumen:
In recent times, some common "non-pathogenic" parasites, such as Blastocystis and Dientamoeba fragilis, have been associated to the aetiology of irritable bowel syndrome (IBS), while host pro-inflammatory cytokine gene polymorphisms might have a role in the pathophysiology of the disease. Therefore, Blastocystis subtypes (ST), D. fragilis and gene promoter single nucleotide polymorphisms of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-a) in IBS patients and controls were studied. After giving written consent, 45 patients with symptoms of IBS according to the Rome III criteria and 45 controls were enrolled. DNAwas extracted from peripheral blood for SNP analysis at position -174 for IL-6 as well as - 238 and -308 for TNF-a. Blastocystis was more common in the IBS group (p=0.043). Interestingly, D. fragilis was found more frequently in the control group (p=0.002); Blastocystis ST1 and 3 were most frequent in both groups. Haploview analysis revealed linkage disequilibrium in TNF-a (p<0.0001); however, none of the SNPs for IL-6 and TNF-a were found to be significantly related with IBS. The clinical and molecular approaches undertaken for the first time in Latin American IBS patients demonstrated an association with Blastocystis that supports a pathogenic role of this parasite in IBS Furthermore, co-infections with ST1 and ST3 were frequent; thus, the genetic diversity proposed within ST polymorphisms does not rule out that particular strains might be associated with disease. In addition, our results do not support a major contribution of IL-6 and TNF-a gene polymorphisms in the susceptibility to IBS. © Springer-Verlag 2011.
Filiaciones:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04536, DF, Mexico
Hospital General Dr. Manuel Gea Gonzalez, Direccion de Investigacion, Laboratorio Clinico, Mexico, DF 14080, Mexico
Department of Microbiology, St.Vincent's Hospital, Darlinghurst 2010 NSW, Australia
Instituto de Ecologia, Universidad Nacional Autonoma de Mexico, Mexico, DF 04510, Mexico
Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico, DF 04536, Mexico
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