Adjuvant immunotherapy of C6 glioma in rats with pertussis toxin
Por:
Orozco-Morales M., Sanchez-Garcia, FJ, Guevara-Salazar P., Arrieta O., Hernández-Pedro N.Y., Sánchez-García A., Perez-Madrigal R., Rangel-López E., Pineda B., Sotelo J.
Publicada:
1 ene 2012
Resumen:
Purpose In spite of the recent advances in surgery and antitumor drugs, the brain tumors, like glioblastoma, have shown a poor prognosis. The aim of this study was to determine the effect of pertussis toxin (PTx) as immunomodulatory molecule on glial tumors induced by C6 glioma cells. Methods Given the pleiotropic effect of PTx on the immune system, we analyzed the effect of PTx on CD4+/CD25+/FoxP3+ (Treg) cells like as immunotherapeutic adjuvant. Thirty rats with a glial tumor of 1.5 cm in diameter were separated in two groups: the first group was treated with PTx and the second group was non-treated (controls). Tumoral volume was measured weekly; tumor, blood and spleen were taken for analysis of subpopulations of T cells, apoptotic index and cytokine contents, in both groups. Results We observed a significant decrease in tumor volume in the PTx group; this was associated with a decreased in the number of Treg cells, in both spleen and tumor. The percentage of apoptotic cells was inc
Filiaciones:
Neuroimmunology Unit, Instituto Nacional de Neurologia y Neurocirugia (INNN), Insurgentes Sur 3877, 14269 Mexico City, Mexico
Immunoregulation Unit from Instituto Politecnico Nacional, Mexico City, Mexico
Laboratory of Experimental Oncology, Instituto Nacional de Cancerologia INCan, Mexico City, Mexico
Pathology Unit, Instituto Nacional de Neurologia y Neurocirugia INNN, Mexico City, Mexico
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