In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species
Por:
Mata-Rosas M., Baltazar-García R.J., Chávez-Avila V.M.
Publicada:
1 ago 2011
Categoría:
Horticulture
Resumen:
A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N(6)-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg-L(-1)) and/or alpha-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg-L(-1) was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg.L(-1) in combination with at NAA 0.1 mg.L(-1). The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without p
Filiaciones:
Mata-Rosas M.:
Instituto de Ecología, A.C, RedManejo Biotecnológico de Recursos, Carretera antigua a Coatepec N 351, El Haya, Xalapa, Veracruz, 91070, Mexico
Baltazar-García R.J.:
Instituto de Ecología, A.C, RedManejo Biotecnológico de Recursos, Carretera antigua a Coatepec N 351, El Haya, Xalapa, Veracruz, 91070, Mexico
Chávez-Avila V.M.:
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
All Open Access; Bronze
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