A helminth cestode parasite express an estrogen-binding protein resembling a classic nuclear estrogen receptor
Por:
Ibarra-Coronado E.G., Escobedo G., Nava-Castro K., Ramses, CRJ, Hernández-Bello R., García-Varela M., Ambrosio J.R., Reynoso-Ducoing O., Fonseca-Liñán R., Ortega-Pierres G., Pavón L., Hernández M.E., Morales-Montor J.
Publicada:
1 sep 2011
Resumen:
The role of an estrogen-binding protein similar to a known mammalian estrogen receptor (ER) is described in the estradiol-dependent reproduction of the helminth parasite Taenia crassiceps. Previous results have shown that 17-beta-estradiol induces a concentration-dependent increase in bud number of in vitro cultured cysticerci. This effect is inhibited when parasites are also incubated in the presence of an ER binding-inhibitor (tamoxifen). RT-PCR assays using specific oligonucleotides of the most conserved ER sequences, showed expression by the parasite of a mRNA band of molecular weight and sequence corresponding to an ER. Western blot assays revealed reactivity with a 66 kDa protein corresponding to the parasite ER protein. Tamoxifen treatment strongly reduced the production of the T. crassiceps ER-like protein. Antibody specificity was demonstrated by immunoprecipitating the total parasite protein extract with anti-ER-antibodies. Cross-contamination by host cells was discarded by f
Filiaciones:
Ibarra-Coronado E.G.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Escobedo G.:
Unidad de Medicina Experimental, Hospital General de México, México D.F. 06726, Mexico
Nava-Castro K.:
Departamento de Infectología e Inmunología, Instituto Nacional de Perinatología, México D.F. 11000, Mexico
Ramses, CRJ:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Hernández-Bello R.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
García-Varela M.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Mexico City 04510, DF, Mexico
Ambrosio J.R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Reynoso-Ducoing O.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Fonseca-Liñán R.:
Departamento de Genética y Biología Molecular, Cinvestav IPN, Av. Instituto Politécnico, Nacional 2508 Col. San Pedro Zacatenco 07360, Mexico
Ortega-Pierres G.:
Departamento de Genética y Biología Molecular, Cinvestav IPN, Av. Instituto Politécnico, Nacional 2508 Col. San Pedro Zacatenco 07360, Mexico
Pavón L.:
Departamento de Psicoinmunología, Instituto Nacional de Psiquiatría Ramón de la Fuente, México D.F., Mexico
Hernández M.E.:
Departamento de Psicoinmunología, Instituto Nacional de Psiquiatría Ramón de la Fuente, México D.F., Mexico
Morales-Montor J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
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