Intrinsic apoptotic pathway is subverted in mouse macrophages persistently infected by RSV
Por:
Nakamura-López Y., Villegas-Sepúlveda N., Sarmiento-Silva R.E., Gómez B.
Publicada:
1 jun 2011
Resumen:
To persist, a virus must co-exist with the host that it infects, thus allowing the virus to survive and to subvert the programmed cell death of the host. In this study, we investigated whether the intrinsic pathway of the apoptotic process is suppressed in a previously reported macrophage cell line persistently infected with respiratory syncytial virus (RSV). To this end, after using staurosporine to induce apoptosis, we determined cell viability and the degree of annexin staining and DNA fragmentation between infected and mock-infected cells. RSV persistence leads to a subversion of apoptosis; whereas in mock-infected macrophages, apoptosis was evident. The cellular apoptotic pathway involve was searched by determining the activities of caspases and the expression of anti-apoptotic proteins. Although caspases-3 and -9 were expressed, their activities were altered; the activity of caspase-3 was reduced and that of caspase-9 could not be detected. Expression of anti-apoptotic proteins B
Filiaciones:
Nakamura-López Y.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Virol Lab, Mexico City 04510, DF, Mexico
Villegas-Sepúlveda N.:
Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del, Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, México, D.F. 07360, Mexico
Sarmiento-Silva R.E.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Virol Lab, Mexico City 04510, DF, Mexico
Gómez B.:
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Virol Lab, Mexico City 04510, DF, Mexico
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