Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the Seeds of White Tepary Bean (Phaseolus Acutifolius Variety Latifolius)
Por:
Valadez-Vega C., Guzmán-Partida A.M., Soto-Cordova F.J., Álvarez-Manilla G., Morales-González J.A., Madrigal-Santillán E., Villagómez-Ibarra J.R., Zúniga-Pérez C., Gutiérrez-Salinas J., Becerril-Flores M.A.
Publicada:
1 mar 2011
Resumen:
The present work shows the characterization of Phaseolus acutifolius variety latifolius, on which little research has been published, and provides detailed information on the corresponding lectin. This protein was purified from a semi-domesticated line of white tepary beans from Sonora, Mexico, by precipitation of the aqueous extract with ammonium sulfate, followed by affinity chromatography on an immobilized fetuin matrix. MALDI TOF analysis of Phaseolus acutifolius agglutinin (PAA) showed that this lectin is composed of monomers with molecular weights ranging between 28 and 31 kDa. At high salt concentrations, PAA forms a dimer of 63 kDa, but at low salt concentrations, the subunits form a tetramer. Analysis of PAA on 2D-PAGE showed that there are mainly three types of subunits with isoelectric points of 4.2, 4.4, and 4.5. The partial sequence obtained by LC/MS/MS of tryptic fragments from the PAA subunits showed 90-100% identity with subunits from genus Phaseolus lectins in previous
Filiaciones:
Valadez-Vega C.:
Institute of Health Sciences, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, 42080 Pachuca de Soto, Hgo, Mexico
Guzmán-Partida A.M.:
Center for Food Research and Development, A. C. Carretera a la Victoria Km 0.6, 83304. Hermosillo, Sonora, Mexico
Soto-Cordova F.J.:
Center for Food Research and Development, A. C. Carretera a la Victoria Km 0.6, 83304. Hermosillo, Sonora, Mexico
Álvarez-Manilla G.:
Ezose Sciences Inc, Pine Brook, NJ 07058, United States
Morales-González J.A.:
Institute of Health Sciences, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, 42080 Pachuca de Soto, Hgo, Mexico
Madrigal-Santillán E.:
Institute of Health Sciences, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, 42080 Pachuca de Soto, Hgo, Mexico
Villagómez-Ibarra J.R.:
Basic Science and Engineering Institute, Universidad Autónoma del Estado de Hidalgo, Carr. A-Pachuca-Tulancingo Km 4.5 Cd Universitaria, 42184, Mineral de la Reforma, Hgo, Mexico
Zúniga-Pérez C.:
Institute of Health Sciences, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, 42080 Pachuca de Soto, Hgo, Mexico
Gutiérrez-Salinas J.:
Laboratory of Biochemistry and Experimental Medicine, Division of Biomedical Research, National Medical Center, 20 de Noviembre, México D.F., Mexico
Becerril-Flores M.A.:
Institute of Health Sciences, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, 42080 Pachuca de Soto, Hgo, Mexico
All Open Access, Gold
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