Modulation by endogenously released ATP and opioids of chromaffin cell calcium channels in mouse adrenal slices
Por:
Hernández A., Segura-Chama P., Jiménez N., García A.G., Hernández-Guijo J.M., Hernández-Cruz A.
Publicada:
1 mar 2011
Resumen:
Hernandez A, Segura-Chama P, Jimenez N, Garcia AG, Hernandez-Guijo JM, Hernandez-Cruz A. Modulation by endogenously released ATP and opioids of chromaffin cell calcium channels in mouse adrenal slices. Am J Physiol Cell Physiol 300: C610-C623, 2011. First published December 15, 2010; doi:10.1152/ajpcell.00380.2010.-Modulation of high-threshold voltage-dependent calcium channels by neurotransmitters has been the subject of numerous studies in cultures of neurons and chromaffin cells. However, no studies on such modulation exist in chromaffin cells in their natural environment, the intact adrenal medullary tissue. Here we performed such a study in voltage-clamped chromaffin cells of freshly prepared mouse adrenal slices under the whole cell configuration of the patch-clamp technique. The subcomponents of the whole cell inward Ca(2+) current (I(Ca)) accounted for 49% for L-, 28% for N-, and 36% for P/Q-type channels. T-type Ca(2+) channels or residual R-type Ca(2+) currents were not seen.
Filiaciones:
Hernández A.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Mexico City 04510, DF, Mexico
Segura-Chama P.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Mexico City 04510, DF, Mexico
Jiménez N.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Mexico City 04510, DF, Mexico
García A.G.:
Instituto Teófilo Hernando, Facultad de Medicina, Universidad Autónoma de Madrid, Av. Arzobispo Morcillo 4, E-28029 Madrid, Spain
Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
Servicio de Farmacología Clínica, IIS Princesa, Universidad Autónoma de Madrid, Madrid, Spain
Hernández-Guijo J.M.:
Instituto Teófilo Hernando, Facultad de Medicina, Universidad Autónoma de Madrid, Av. Arzobispo Morcillo 4, E-28029 Madrid, Spain
Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain
Hernández-Cruz A.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Mexico City 04510, DF, Mexico
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