Saccharomyces cerevisiae Bat1 and Bat2 Aminotransferases Have Functionally Diverged from the Ancestral-Like Kluyveromyces lactis Orthologous Enzyme
Por:
Colon, M, Hernandez, F, Lopez, K, Quezada, H, Gonzalez, J, Lopez, G, Aranda, C, Gonzalez, A
Publicada:
18 ene 2011
Resumen:
Background: Gene duplication is a key evolutionary mechanism providing material for the generation of genes with new or modified functions. The fate of duplicated gene copies has been amply discussed and several models have been put forward to account for duplicate conservation. The specialization model considers that duplication of a bifunctional ancestral gene could result in the preservation of both copies through subfunctionalization, resulting in the distribution of the two ancestral functions between the gene duplicates. Here we investigate whether the presumed bifunctional character displayed by the single branched chain amino acid aminotransferase present in K. lactis has been distributed in the two paralogous genes present in S. cerevisiae, and whether this conservation has impacted S. cerevisiae metabolism. Principal Findings: Our results show that the KlBat1 orthologous BCAT is a bifunctional enzyme, which participates in the biosynthesis and catabolism of branched chain ami
Filiaciones:
Colon, M:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Hernandez, F:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Lopez, K:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Gonzalez, J:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Lopez, G:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Aranda, C:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Gonzalez, A:
Univ Nacl Autonoma Mexico, Dept Bioquim & Biol Estruct, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
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