Entamoeba histolytica calreticulin: an endoplasmic reticulum protein expressed by trophozoites into experimentally induced amoebic liver abscesses
Por:
González E., de Leon, MDG, Meza I., Ocadiz-Delgado R., Gariglio P., Silva-Olivares A., Galindo-Gómez S., Shibayama M., Morán P., Valadez A., Limón A., Rojas L., Hernández E.G., Cerritos R., Ximenez C.
Publicada:
1 feb 2011
Resumen:
Entamoeba histolytica calreticulin (EhCRT) is remarkably immunogenic in humans (90-100% of invasive amoebiasis patients). Nevertheless, the study of calreticulin in this protozoan is still in its early stages. The exact location, biological functions, and its role in pathogenesis are yet to be fully understood. The aim of the present work is to determine the location of EhCRT in virulent trophozoites in vivo and the expression of the Ehcrt gene during the development of experimentally induced amoebic liver abscesses (ALA) in hamsters. Antibodies against recombinant EhCRT were used for the immunolocalization of EhCRT in trophozoites through confocal microscopy; immunohistochemical assays were also performed on tissue sections of ALAs at different times after intrahepatic inoculation. The expression of the Ehcrt gene during the development of ALA was estimated through both in situ RT-PCR and real-time RT-PCR. Confocal assays of virulent trophozoites showed a distribution of EhCRT in the
Filiaciones:
González E.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
de Leon, MDG:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Meza I.:
Depto. de Biomedicina Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Ocadiz-Delgado R.:
Depto. de Genética Y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Gariglio P.:
Depto. de Genética Y Biología Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Silva-Olivares A.:
Depto. de Infectómica Y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Galindo-Gómez S.:
Depto. de Infectómica Y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Shibayama M.:
Depto. de Infectómica Y Patogénesis Molecular, CINVESTAV, Ave. Politécnico Nacional No 2508, CP 07360 México, D.F., Mexico
Morán P.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Valadez A.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Limón A.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Rojas L.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Hernández E.G.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Cerritos R.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Ximenez C.:
Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
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