Anti-11[E]-pyroglutamate-modified amyloid beta antibodies cross-react with other pathological A beta species: Relevance for immunotherapy
Por:
Perez-Garmendia R., Ibarra-Bracamontes V., Vasilevko V., Luna-Muñoz J., Mena R., Govezensky T., Acero G., Manoutcharian K., Cribbs D.H., Gevorkian G.
Publicada:
15 dic 2010
Resumen:
N-truncated/modified forms of amyloid beta (A beta) peptide are found in diffused and dense core plaques in Alzheimer's disease (AD) and Down's syndrome patients as well as animal models of AD, and represent highly desirable therapeutic targets. In the present study we have focused on N-truncated/modified A beta peptide bearing amino-terminal pyroglutamate at position 11 (A beta N11(pE)). We identified two B-cell epitopes recognized by rabbit anti-A beta N11(pE) polyclonal antibodies. Interestingly, rabbit anti-A beta N11(pE) polyclonal antibodies bound also to full-length A beta 1-42 and N-truncated/modified A beta N3(pE), suggesting that the three peptides may share a common B-cell epitope. Importantly, rabbit anti-A beta N11(pE) antibodies bound to naturally occurring A beta aggregates present in brain samples from AD patients. These results are potentially important for developing novel immunogens for targeting N-truncated/modified A beta aggregates as well, since the most commonly
Filiaciones:
Perez-Garmendia R.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Ibarra-Bracamontes V.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Vasilevko V.:
The Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697-4540, United States
Luna-Muñoz J.:
Department of Neurosciences, CINVESTAV-IPN, DF, Mexico
Mena R.:
Department of Neurosciences, CINVESTAV-IPN, DF, Mexico
Govezensky T.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Acero G.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Manoutcharian K.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Cribbs D.H.:
The Institute for Memory Impairments and Neurological Disorders, University of California Irvine, Irvine, CA 92697-4540, United States
Department of Neurology, University of California Irvine, Irvine, CA 92697-4540, United States
Gevorkian G.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
All Open Access; Green
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