Neuronal bursting properties in focal and parafocal regions in pediatric neocortical epilepsy stratified by histology
Por:
Marcuccilli C.J., Tryba A.K., Van Drongelen W., Koch H., Viemari J.C., Pena-Ortega, F, Doren E.L., Pytel P., Chevalier M., Mrejeru A., Kohrman M.H., Lasky R.E., Lew S.M., Frim D.M., Ramirez, JM
Publicada:
1 dic 2010
Resumen:
To test the hypothesis that focal and parafocal neocortical tissue from pediatric patients with intractable epilepsy exhibits cellular and synaptic differences, the authors characterized the propensity of these neurons to generate (a) voltage-dependent bursting and (b) synaptically driven paroxysmal depolarization shifts. Neocortical slices were prepared from tissue resected from patients with intractable epilepsy. Multiunit network activity and simultaneous whole-cell patch recordings were made from neurons from three patient groups: (1) those with normal histology; (2) those with mild and severe cortical dysplasia; and (3) those with abnormal pathology but without cortical dysplasia. Seizure-like activity was characterized by population bursting with concomitant bursting in intracellularly recorded cortical neurons (n = 59). The authors found significantly more N-methyl-d-aspartic acid-driven voltage-dependent bursting neurons in focal versus parafocal tissue in patients with severe cortical dysplasia (P < 0.01). Occurrence of paroxysmal depolarization shifts and burst amplitude and burst duration were significantly related to tissue type: focal or parafocal (P < 0.05). The authors show that functional differences between focal and parafocal tissue in patients with severe cortical dysplasia exist. There are functional differences between patient groups with different histology, and bursting properties can be significantly associated with the distinction between focal and parafocal tissue. Copyright © 2010 by the American Clinical Neurophysiology Society.
Filiaciones:
Marcuccilli C.J.:
Department of Neurology, Medical College of Wisconsin, 9000 W. Wisconsin Ave., Milwaukee, WI 53201, United States
Department of Neurosurgery, Medical College of Wisconsin, Milwaukee, WI, United States
Tryba A.K.:
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States
Van Drongelen W.:
Department of Pediatrics, University of Chicago, Chicago, IL, United States
Koch H.:
Department of Neurological Surgery, Seattle Children's Research Institute, University of Washington, Seattle, WA, United States
Center for Integrative Brain Research, Seattle Children's Research Institute, University of Washington, Seattle, WA, United States
Viemari J.C.:
Laboratoire Plasticité et Physio-Pathologie de la Motricité, UMR 6196, CNRS-Aix Marseille University, Marseille, France
Pena-Ortega, F:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Dept Neurobiol Desarrollo & Neurofisiol, Queretaro, Mexico
Doren E.L.:
Department of Pediatrics, University of Chicago, Chicago, IL, United States
Pytel P.:
Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL, United States
Chevalier M.:
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States
Mrejeru A.:
Department of Pathology, University of Chicago, Chicago, IL, United States
Kohrman M.H.:
Department of Pediatrics, University of Chicago, Chicago, IL, United States
Lasky R.E.:
Center for Clinical Research and Evidence-Based Medicine, University of Texas-Houston Medical School, Houston, TX, United States
Lew S.M.:
Department of Neurosurgery, Medical College of Wisconsin, Milwaukee, WI, United States
Frim D.M.:
Department of Neurosurgery, University of Chicago, Chicago, IL, United States
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