Evaluation of Bacillus thuringiensis pathogenicity for a strain of the tick, Rhipicephalus microplus, resistant to chemical pesticides
Por:
Fernández-Ruvalcaba M., Pena-Chora, G, Romo-Martínez A., Hernández-Velázquez V., De La Parra A.B., De La Rosa D.P.
Publicada:
22 oct 2010
Categoría:
Insect Science
Resumen:
The pathogenicity of four native strains of Bacillus thuringiensis against Rhipicephalus (Boophilus) microplus (Canestrine) (Acari: Ixodidae) was evaluated. A R. microplus strain that is resistant to organophosphates, pyrethroids, and amidines, was used in this study. Adult R. microplus females were bioassayed using the immersion test of Drummond against 60 B. thuringiensis strains. Four strains, GP123, GP138, GP130, and GP140, were found to be toxic. For the immersion test, the total protein concentration for each bacterial strain was 1.25 mg/ml. Mortality, oviposition, and egg hatch were recorded. All of the bacterial strains had significant effects compared to the controls, but no significant differences were seen between the 4 strains. It is evident that these B. thuringiensis strains have a considerable detrimental effect on the R. microplus strain that is resistant to pesticides.
Filiaciones:
Fernández-Ruvalcaba M.:
Centro Nacional de Investigaciones en Parasitología Veterinaria INIFAP, Km. 11.5 Carretera Federal Cuernavaca-Cuautla, Col. Progreso Jiutepec, Morelos, Mexico, C. P. 62550, Mexico
Romo-Martínez A.:
Centro Nacional de Investigaciones en Parasitología Veterinaria INIFAP, Km. 11.5 Carretera Federal Cuernavaca-Cuautla, Col. Progreso Jiutepec, Morelos, Mexico, C. P. 62550, Mexico
Hernández-Velázquez V.:
Centro de Investigaciones en Biotecnología, Universidad Autónoma Del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, Mexico, C. P. 62209, Mexico
De La Parra A.B.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
De La Rosa D.P.:
Centro Nacional de Investigaciones en Parasitología Veterinaria INIFAP, Km. 11.5 Carretera Federal Cuernavaca-Cuautla, Col. Progreso Jiutepec, Morelos, Mexico, C. P. 62550, Mexico
|