Identification and characterization of a surface-associated, subtilisin-like serine protease in Trichomonas vaginalis
Por:
Hernández-Romano P., Hernández R., Arroyo R., Alderete J.F., López-Villaseñor I.
Publicada:
1 sep 2010
Resumen:
Trichomonas vaginalis is a protozoan parasite causing trichomonosis, a sexually transmitted infection in humans. This parasite has numerous proteases, most of which are cysteine proteases that appear to be involved in adherence and cytotoxicity of host cells. In this report we identify and characterize a putative subtilisin-like serine protease (SUB1). The sub1 gene encodes a 101-kDa protein. In silico analyses predict signal and pro-peptides at the N-terminus, and a transmembrane helix at the carboxy-terminal region. The sub1 gene was found as single copy by Southern analysis, albeit additional serine protease related genes are annotated in the T. vaginalis genome. The expression of sub1 could only be detected by RT-PCR and Ribonuclease Protection Assays, suggesting a low abundant mRNA. The sub1 gene transcription start site was correctly assigned by RPA. The transcript abundance was found to be modulated by the availability of iron in the growth medium. Antibodies raised to a specifi
Filiaciones:
Hernández-Romano P.:
Univ Nacl Autonoma Mexico, Dept Mol Biol & Biotecnol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Hernández R.:
Univ Nacl Autonoma Mexico, Dept Mol Biol & Biotecnol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Arroyo R.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigacin y de Estudios Avanzados Del IPN (CINVESTAV-IPN), México D.F., Mexico
Alderete J.F.:
School of Molecular Biosciences, Washington State University, Pullman, WA, United States
López-Villaseñor I.:
Univ Nacl Autonoma Mexico, Dept Mol Biol & Biotecnol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
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