The Hemorrhagic Coli Pilus (HCP) of Escherichia coli O157:H7 Is an Inducer of Proinflammatory Cytokine Secretion in Intestinal Epithelial Cells
Por:
Ledesma M.A., Ochoa S.A., Cruz A., Rocha-Ramírez L.M., Mas-Oliva J., Eslava C.A., Girón J.A., Xicohtencatl-Cortes J.
Publicada:
12 ago 2010
Resumen:
Background: Enterohemorrhagic Escherichia coli (EHEC) O157:H7, the causative agent of hemorrhagic colitis and the hemolytic uremic syndrome (HUS), produces long bundles of type IV pili (TFP) called hemorrhagic coli pili (HCP). HCP are capable of mediating several phenomena associated with pathogenicity: i) adherence to human and bovine epithelial cells; ii) invasion of epithelial cells; iii) hemagglutination of rabbit erythrocytes; iv) biofilm formation; v) twitching motility; and vi) specific binding to laminin and fibronectin. HCP are composed of a 19 kDa pilin subunit (HcpA) encoded by the hcpA chromosomal gene (called prepilin peptidase-dependent gene [ppdD] in E. coli K-12). Methodology/Principal Findings: In this study we investigated the potential role of HCP of E. coli O157:H7 strain EDL933 in activating the release of pro-and anti-inflammatory cytokines from a variety of host epithelial cells. We found that purified HCP and a recombinant HcpA protein induced significant releas
Filiaciones:
Ledesma M.A.:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, México Distrito Federal, Mexico
Ochoa S.A.:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, México Distrito Federal, Mexico
Cruz A.:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, México Distrito Federal, Mexico
Rocha-Ramírez L.M.:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, México Distrito Federal, Mexico
Mas-Oliva J.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Eslava C.A.:
Univ Nacl Autonoma Mexico, Dept Salud Publ, Fac Med, Mexico City 04510, DF, Mexico
Girón J.A.:
Department of Molecular Genetics and Microbiology, Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States
Xicohtencatl-Cortes J.:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, México Distrito Federal, Mexico
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