High-throughput profiling of the humoral immune responses against thirteen human papillomavirus types by proteome microarrays
Por:
Luevano M., Bernard, HU, Barrera-Saldaña H.A., Trevino V., Garcia-Carranca A., Villa L.L., Monk B.J., Tan X., Davies D.H., Felgner P.L., Kalantari M.
Publicada:
15 sep 2010
Categoría:
Virology
Resumen:
We have developed microarrays with all eight proteins encoded by 13 different human papillomavirus types associated with anogenital cancer (HPV-16, -18, -31. -33, -35, -45, and -53), genital warts (HPV-6 and -11), or skin lesions (HPV-1. -2, -4. and -5) We analyzed the seroprevalence of antibodies in 546 patients, which had either cervical carcinomas, or precursor lesions, or which were asymptomatic All patient groups contained sera ranging from high reactivity against multiple HPV proteins to low or no reactivity Computational analyses showed the E7 proteins of carcinogenic HPV types as significantly more reactive in cancer patients compared to asymptomatic individuals and discriminating between cancel and HSIL or LSIL patients Antibodies against E4 and E5 had the highest seroprevalence but did not exhibit differential reactivity relative to pathology Our study introduces a new approach to future evaluation of the overall antigenicity of HPV proteins and cross-reaction between homolog
Filiaciones:
Luevano M.:
Departamento de Bioquimica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico
Barrera-Saldaña H.A.:
Departamento de Bioquimica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico
Trevino V.:
Ingenieria Biomedicas, Instituto Tecnologico y de Estudios Superiores de Monterrey, Nuevo Leon, Mexico
Garcia-Carranca A.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Villa L.L.:
Ludwig Institute for Cancer Research, São Paulo, Brazil
Monk B.J.:
Department of Obstetrics and Gynecology, University of California Irvine, Irvine, CA 92697, United States
Tan X.:
Department of Medicine, University of California Irvine, Irvine, CA 92697, United States
Davies D.H.:
Department of Medicine, University of California Irvine, Irvine, CA 92697, United States
Felgner P.L.:
Department of Medicine, University of California Irvine, Irvine, CA 92697, United States
Kalantari M.:
Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, United States
All Open Access, Bronze
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