Actin Cytoskeleton Participation in the Onset of IL-1 beta Induction of an Invasive Mesenchymal-like Phenotype in Epithelial MCF-7 Cells
Por:
Franco-Barraza J., Valdivia-Silva J.E., Zamudio-Meza H., Castillo A., García-Zepeda E.A., Benítez-Bribiesca L., Meza I.
Publicada:
1 abr 2010
Categoría:
Medicine (miscellaneous)
Resumen:
Background. Interleukin 1 beta (IL-1 beta) and other inflammatory cytokines are reported to induce phenotypic changes in epithelial breast cancer tumor cells related to increased invasiveness. Mechanisms involved in the process are not well understood. Methods. The noninvasive breast cancer epithelial cell line MCF-7 was used to investigate the IL-1 beta-induced phenotype. Live cells expressing EGFP-actin were monitored for cell morphology changes and actin cytoskeleton dynamics by time-lapse video microscopy in the presence of IL-1 beta and specific inhibitors of actin signaling pathways. Chemotaxis, invasion of Matrigel, MMP activity and expression of S100A4 in cells treated with IL-1 beta were assessed by migration assays, zymograms and immunoblots. Results. Exposure to IL-1 beta specifically induced a change in MCF-7 cells from a typical epithelial morphology into elongated cells, showing numerous dynamic actin-rich lamellae and peripheral ruffles characteristic of fibroblasts. The
Filiaciones:
Franco-Barraza J.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Mexico, Mexico
Valdivia-Silva J.E.:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Zamudio-Meza H.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Mexico, Mexico
Castillo A.:
Departmento de Fisiologia, CINVESTAV-IPN, Mexico, Mexico
García-Zepeda E.A.:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Benítez-Bribiesca L.:
Unidad de Investigacion Medica de Enfermedades Oncologicas, CMN-SXXI IMSS, Mexico, Mexico
Meza I.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Mexico, Mexico
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