Induction of peroxisomal proliferator-activated receptor gamma and peroxisomal proliferator-activated receptor gamma coactivator 1 by unsaturated fatty acids, retinoic acid, and
Por:
García-Rojas P., Antaramian A., González-Dávalos L., Villarroya F., Shimada A., Varela-Echavarría A., Mora O.
Publicada:
1 may 2010
Resumen:
The importance of dietary fat components, such as fatty acids, in the expression of multiple genes is clear. In the case of beef cattle, fat in the form of fatty acids (saturated or unsaturated), vitamin A (mainly retinoic acid), or carotenoids (beta-carotene and lutein) is obtained from dietary feed or pasture. The aim of this work was to study the effect of fatty acids (phytanic and pristanic acids), vitamin A (all-trans and 9-cis retinoic acid), and carotenoids (beta-carotene and lutein) on the expression of PPAR. and its coactivator PGC-1 alpha during differentiation of bovine white adipose tissue. Samples were collected at slaughter from subcutaneous adipose tissue and processed in a solution containing type II collagenase for 2 h at 37 degrees C. Cells were resuspended in basal medium, Dulbecco's modified Eagle's medium containing 5% fetal bovine serum, plated on 24-well culture plates at a density of 1 x 10(4) cells/cm(2), and incubated at 37 degrees C in a 5% CO(2) atmosphere.
Filiaciones:
Univ Nacl Autonoma Mexico, Programa Posgrado Ciencias Prod & Salud Anim, Mexico City 04510, DF, Mexico
UNAM, Inst Neurobiol, Queretaro 76230, Qro, Mexico
UNAM, Fac Estudios Super Cuautitlan, Secretaria Posgrado, Lab Rumiol & Metab Nutr, Queretaro 76140, Qro, Mexico
Departamento de Bioquímica y Biología Molecular e Instituto de Biomedicina (IBUB), Universitat de Barcelona y CIBER Fisiopatología de la Obesidad y Nutrición, Av. Diagonal 645, Barcelona, 08028, Spain
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