Sodium caseinate induces secretion of macrophage colony-stimulating factor from neutrophils
Por:
Santiago-Osorio, E, Mora, L, Bautista, M, Montesinos, JJ, Martinez, I, Ramos-Mandujano, G, Zambrano, R, Monroy-Garcia, A, Weiss-Steider, B, Ledesma-Martinez, E, Aguiniga, I
Publicada:
1 abr 2010
Resumen:
In this work we provide evidence that granulocytes produce macrophage colony-stimulating factor (M-CSF) in the band cell stage and secrete it upon sodium caseinate-mediated differentiation to polymorphonuclear cells. We identified M-CSF in an enriched population of myeloid band cells from murine bone marrow using a chromophore-labeled monoclonal anti-M-CSF antibody. An ELISA assay was then used to detect secreted M-CSF in culture supernatants of enriched band cells differentiated to mature neutrophils using sodium caseinate. Colony formation in vitro by the supernatants from differentiating band cells was blocked by anti-M-CSF, thus suggesting that this factor is the only one responsible for this activity. Our data imply that casein can modulate hematopoiesis possibly via M-CSF production. Finally we discuss the possibility whether this M-CSF in concert with G-CSF could establish a cellular communication network between macrophages and granulocytes allowing them to simultaneously arriv
Filiaciones:
Santiago-Osorio, E:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Mora, L:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Bautista, M:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Montesinos, JJ:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Martinez, I:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Ramos-Mandujano, G:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Zambrano, R:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Monroy-Garcia, A:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Weiss-Steider, B:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Ledesma-Martinez, E:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
Aguiniga, I:
Univ Nacl Autonoma Mexico, Lab Hematopoyesis & Leucemia, PB,Lab 8, Fac Estudies Super Zaragoza, Mexico City 09230, DF, Mexico
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