Phylogenetic analysis of nuclear and mitochondrial DNA reveals a complex of cryptic species in Crassicutis cichlasomae (Digenea: Apocreadiidae), a parasite of Middle-American ci
Por:
Razo-Mendivil U., Vázquez-Domínguez E., Rosas-Valdez R., de Leon, GPP, Nadler S.A.
Publicada:
15 mar 2010
Resumen:
We obtained nuclear ITS-1 and mitochondrial cox1 sequences from 225 Crassicutis cichlasomae adults collected in 12 species of cichlids from 32 localities to prospect for the presence of cryptic species. This trematode is commonly found in species of cichlids over a wide geographic range in Middle-America. Population-level phylogenetic analyses of ITS-1 and cox1, assessments of genetic and haplotype diversity, and morphological observations revealed that C. cichlasomae represents a complex of seven cryptic species for which no morphological diagnostic characters have been discovered thus far. Bayesian and Maximum Likelihood analyses of concatenated datasets (906 bp) recovered eight lineages of C. cichlasomae, all with high posterior probabilities and bootstrap branch support. Values of genetic divergence between clades ranged from 1.0% to 5.2% for ITS-1, and from 7.2% to 30.0% for cox1. Morphological study of more than 300 individuals did not reveal structural diagnostic traits for the
Filiaciones:
Razo-Mendivil U.:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Vázquez-Domínguez E.:
Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico
Rosas-Valdez R.:
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
de Leon, GPP:
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
Nadler S.A.:
Department of Nematology, University of California, One Shields Avenue, Davis, CA 95616-8668, United States
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