A New MAP Kinase Protein Involved in Estradiol-Stimulated Reproduction of the Helminth Parasite Taenia crassiceps
Por:
Escobedo G., Soldevila G., Ortega-Pierres G., Chávez-Ríos J.R., Nava K., Fonseca-Liñán R., López-Griego L., Hallal-Calleros C., Ostoa-Saloma P., Morales-Montor J.
Publicada:
1 ene 2010
Resumen:
MAP kinases (MAPK) are involved in the regulation of cellular processes such as reproduction and growth. In parasites, the role of MAPK has been scarcely studied. Here, we describe the participation of an ERK-like protein in estrogen-dependent reproduction of the helminth parasite Taenia crassiceps. Our results show that 17 beta-estradiol induces a concentration-dependent increase in the bud number of in vitro cultured cysticerci. If parasites are also incubated in presence of an ERK-inhibitor, the stimulatory effect of estrogen is blocked. The expression of ERK-like mRNA and its corresponding protein was detected in the parasite. The ERK-like protein was over-expressed by all treatments. Nevertheless, a strong induction of phosphorylation of this protein was observed only in response to 17 beta-estradiol. Cross-contamination by host cells was discarded by flow cytometry analysis. Parasite cells expressing the ERK-like protein were exclusively located at the subtegument tissue by confo
Filiaciones:
Escobedo G.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, DF, Mexico
Soldevila G.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Ortega-Pierres G.:
Departamento de Genética y Biología Molecular, Cinvestav, IPN, Av. Inst. Pol. Nac. 2508 Col. San Pedro Zacatenco, México D.F. 07360, Mexico
Chávez-Ríos J.R.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Nava K.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Fonseca-Liñán R.:
Departamento de Genética y Biología Molecular, Cinvestav, IPN, Av. Inst. Pol. Nac. 2508 Col. San Pedro Zacatenco, México D.F. 07360, Mexico
López-Griego L.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Hallal-Calleros C.:
Facultad de Farmacia, Universidad Autónoma Del Estado de Morelos, Morelos 62209, Mexico
Ostoa-Saloma P.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Morales-Montor J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Gold
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