Transformed Cell Suspension Culture of Galphimia glauca Producing Sedative Nor-friedelanes
Por:
Ortíz A., Cardoso-Taketa A., Monroy M.R., Arellano J., Hernández G., Villarreal M.L.
Publicada:
1 mar 2010
Resumen:
The Mexican species Galphimia glauca (Cav.) Kuntze (Malphigiaceae) synthesises a family of sedative and anxiolytic nor-secofriedelanes, designated as galphimines. These active principles accumulate at low concentration in the aerial parts of plants from wild populations. Transformed calluses and cell suspension cultures of this species were established in order to induce a greater production of nor-friedelanes. The cell suspension line GgBa was selected and grown over a period of two years of continuous subculturing in MS nutrient medium in the absence of growth regulators. PCR and Southern blot analyses were employed in order to confirm that the rol A gene had been integrated into the plant genome. Batch cultures of the GgBa cell line were grown over a 32-day period and first-order growth kinetics was observed, reaching a specific growth rate (mu) of 0.13 d(-1). The production of glaucacetalin A (10), a triterpenoid related to the known galphimines, was quantified in the nutrient medi
Filiaciones:
Ortíz A.:
Centro de Investigación en Biotecnología, Universidad Autónoma Del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, 62209 Morelos, Mexico
Cardoso-Taketa A.:
Centro de Investigación en Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, Mexico
Monroy M.R.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62191, Morelos, Mexico
Arellano J.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62191, Morelos, Mexico
Hernández G.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62191, Morelos, Mexico
Villarreal M.L.:
Centro de Investigación en Biotecnología, Universidad Autónoma Del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, 62209 Morelos, Mexico
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