Isolation of jacaranone, a sedative constituent extracted from the flowers of the Mexican tree Ternstroemia pringlei
Por:
Lozada-Lechuga J., Villarreal M.L., Fliniaux, MA, Bensaddek L., Mesnard F., Gutierrez, MD, Cardoso-Taketa A.T.
Publicada:
3 feb 2010
Resumen:
Ethnopharmacological relevance: Ternstroemia pringlei represents one of the most widely employed and commercially exploited medicinal plant in Mexico, used popularly as a tranquilizer and for the treatment of insomnia. Aim of the study: To investigate the sedative constituents of the plant through a bio-guided fractionation of extracts derived from calyx and fruits. Materials and methods: Crude extracts with different polarities (CHCl3, AcOEt, MeOH, aqueous) were prepared and subjected to chromatographic fractionation, leading to the isolation of the sedative compound (1) from the MeOH crude extract. The identity of 1 was unequivocally established by means of 1D and 2D NMR spectroscopic analysis. The sleeping time induced by sodium pentobarbital and the elevated plus-maze models were performed on mice to determine the sedative and anxiolytic activities, respectively. Bioactivity was also investigated though in vitro GABA release experiments using mice brain slices. Results:The sedative
Filiaciones:
Lozada-Lechuga J.:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico
Villarreal M.L.:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico
Bensaddek L.:
Faculté de Pharmacie, Université de Picardie Jules Verne, Unité de Recherche EA3900 - BioPI, 1 rue des Louvels, F80 037 Amiens, France
Mesnard F.:
Faculté de Pharmacie, Université de Picardie Jules Verne, Unité de Recherche EA3900 - BioPI, 1 rue des Louvels, F80 037 Amiens, France
Cardoso-Taketa A.T.:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico
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