Expression and immunolocalization of ERG1 potassium channels in the rat kidney
Por:
Carrisoza R., Salvador C., Bobadilla N.A., Trujillo J., Escobar L.I.
Publicada:
1 feb 2010
Resumen:
Potassium (K(+)) channels participate in K(+) secretion, K(+) recycling, and cell volume regulation and help to maintain the resting potential in mammalian kidneys. Previously, we identified a set of voltage-gated K(+) channels (Kv1) in the inner medullary collecting duct of the rat kidney. In the present work, we identified the voltage-gated K(+) channel ether-A -go-go-related gene (ERG) in the rat kidney. mRNAs of ERG1a and its N-terminal splice-variant ERG1b were detected. Immunoblots of the cortex and medulla revealed two molecular mass proteins of 135 and 80 kDa, consistent in size with the nonglycosylated ERG1a and ERG1b isoforms, respectively. However, bands of 155 and 95 kDa, corresponding to mature glycosylated ERG1a and ERG1b, respectively, were also observed. In our immunohistochemical experiments, we could not differentiate the ERG1 isoforms because we used an antibody against a carboxy-terminal epitope. ERG1 was differentially localized in specific nephron segments: its lo
Filiaciones:
Carrisoza R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, Mexico City 04510, DF, Mexico
Salvador C.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, Mexico City 04510, DF, Mexico
Bobadilla N.A.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Unidad Fisiol Mol, Mexico City 04510, DF, Mexico
Trujillo J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Unidad Fisiol Mol, Mexico City 04510, DF, Mexico
Escobar L.I.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, Mexico City 04510, DF, Mexico
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