Cross-talk between Rac1 and Cdc42 GTPases regulates formation of filopodia required for dengue virus type-2 entry into HMEC-1 cells
Por:
Zamudio-Meza H., Castillo-Alvarez A., González-Bonilla C., Meza I.
Publicada:
1 dic 2009
Categoría:
Virology
Resumen:
Infection with dengue virus type-2 (DENV-2) begins with virus adherence to cell surface receptors. In endothelial cells (HMEC-1), a cell model for DENV-2 infection, alpha 5 beta 3 integrin has been identified as a putative receptor for the virus. Previous work had suggested that the actin cytoskeleton of HMEC-1 cells plays an important role in virus entry and infection. In the present work, fixed and living HMEC-1 cells expressing enhanced green fluorescent protein-actin were monitored for actin reorganization after virus inoculation, utilizing fluorescence and time lapse microscopy. Cell infection and production of infective viruses were quantified using an anti-E protein antibody and by measuring the p.f.u. ml(-1). Specific drugs that antagonize actin organization and regulate actin-signalling pathways were tested in viral adhesion and infection assays, as were the expression of dominant-negative Rac1 and Cdc42 proteins. Disorganization of actin precluded infection, while microtubule
Filiaciones:
Zamudio-Meza H.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Apartado 14-740, México, DF 07360, Mexico
Castillo-Alvarez A.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Apartado 14-740, México, DF 07360, Mexico
González-Bonilla C.:
Unidad de Investigación Biomed´ica, Centro Médico Nacional La Raza-IMSS, México, DF, Mexico
Meza I.:
Departamento de Biomedicina Molecular, CINVESTAV-IPN, Apartado 14-740, México, DF 07360, Mexico
All Open Access, Bronze
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