DETECTING A COMPLEX OF CRYPTIC SPECIES WITHIN NEOECHINORHYNCHUS GOLVANI (ACANTHOCEPHALA: NEOECHINORHYNCHIDAE) INFERRED FROM ITSs AND LSU rDNA GENE SEQUENCES
Por:
Martinez-Aquino, A, Reyna-Fabian, ME, Rosas-Valdez, R, Razo-Mendivil, U, de Leon, GPP, Garcia-Varela, M
Publicada:
1 oct 2009
Resumen:
Neoechinorhynchus golvani is an intestinal parasite of freshwater and brackish water fishes distributed in Mexico. The genetic variability of 40 samples representing 12 populations from north, south, and central Mexico, and I from Costa Rica, was estimated by sequencing 2 nuclear genes (ITS1, 5.8S, ITS2, and LSU rDNA, including the domain D2 + D3). The length of both genes ranged from 700 to 779 base pairs (bp) and from 813, to 821 bp, for ITSs Lind LSU, respectively. The genetic divergence among populations ranged from 19.5 to 35.3% with ITSs and from 9.28 to 19.58% with LSU. Maximum likelihood and maximum parsimony analyses were performed for each data set and also for 2 combined data sets (ITSs + LSU rDNA with and without outgroups). showing strong similarities among trees, with high bootstrap support in all cases. Genetic divergence, in combination with phylogenetic analyses, Suggested that the acanthocephalan N. golvani represents a complex of cryptic species, which is composed of
Filiaciones:
Martinez-Aquino, A:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
Reyna-Fabian, ME:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
Rosas-Valdez, R:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
Razo-Mendivil, U:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
de Leon, GPP:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
Garcia-Varela, M:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Mexico City 04510, DF, Mexico
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