Characterization of recombinant human cementum protein 1 (hrCEMP1): Primary role in biomineralization
Por:
Villarreal-Ramírez E., Moreno A., Mas-Oliva J., Chávez-Pacheco J.L., Narayanan A.S., Gil-Chavarría I., Zeichner-David M., Arzate H.
Publicada:
19 jun 2009
Resumen:
Cementum protein 1 (CEMP1) has been recently cloned, and in vitro experiments have shown functions as regulator of cementoblast behavior and inducer of differentiation of non-osteogenic cells toward a cementoblastic/osteoblastic phenotype. In this study, we have produced a full-length human recombinant CEMP1 protein in a human gingival fibroblast cell line. The purified protein (hrCEMP1) has a M, 50,000. Characterization of hrCEMP1 indicates that its secondary Structure is mainly composed of beta-sheet (55%), where random coil and alpha helix conformations correspond to 35% and 10%, respectively. It was found that hrCEMP1 is N-glycosylated, phosphorylated and possesses strong affinity for hydroxyapatite. Even more important, our results show that hrCEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate (OCP) crystal nucleation. These features make CEMP1 a very good candidate for biotechnological applications in order to achieve cementum and/or bone r
Filiaciones:
Villarreal-Ramírez E.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Biol Periodontal & Tejidos Mineralizados, Mexico City 04510, DF, Mexico
Moreno A.:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
Mas-Oliva J.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Chávez-Pacheco J.L.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Biol Periodontal & Tejidos Mineralizados, Mexico City 04510, DF, Mexico
Narayanan A.S.:
Department of Pathology, School of Medicine, University of Washington, Seattle, 98195, United States
Gil-Chavarría I.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Biol Periodontal & Tejidos Mineralizados, Mexico City 04510, DF, Mexico
Zeichner-David M.:
Center for Craniofacial Molecular Biology, University of Southern California, United States
Arzate H.:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Biol Periodontal & Tejidos Mineralizados, Mexico City 04510, DF, Mexico
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