Mycobacterium tuberculosis Glycoproteomics Based on ConA-Lectin Affinity Capture of Mannosylated Proteins
Por:
González-Zamorano M., Mendoza-Hernandez, G, Xolalpa W., Parada C., Vallecillo A.J., Bigi F., Espitia C.
Publicada:
1 feb 2009
Resumen:
A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico
Instituto de Biotecnología, CICVyA-INTA Castelar, Los Reseros y Las Cabanas Bl 712WAA Hurlingham, Argentina
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