CLB19, a pentatricopeptide repeat protein required for editing of rpoA and clpP chloroplast transcripts
Por:
Chateigner-Boutin, AL, Ramos-Vega M., Guevara-García A., Andrés C., Gutierrez-Nava, MD, Cantero A., Delannoy E., Jiménez L.F., Lurin C., Small I., Leo P.
Publicada:
1 nov 2008
Resumen:
RNA editing changes the sequence of many transcripts in plant organelles, but little is known about the molecular mechanisms determining the specificity of the process. In this study, we have characterized CLB19 (also known as PDE247), a gene that is required for editing of two distinct chloroplast transcripts, rpoA and clpP. Loss-of-function clb19 mutants present a yellow phenotype with impaired chloroplast development and early seedling lethality under greenhouse conditions. Transcript patterns are profoundly affected in the mutant plants, with a pattern entirely consistent with a defect in activity of the plastid-encoded RNA polymerase. CLB19 encodes a pentatricopeptide repeat protein similar to the editing specificity factors CRR4 and CRR21, but, unlike them, is implicated in editing of two target sites.
Filiaciones:
Ramos-Vega M.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62271, Morelos, Mexico
Guevara-García A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62271, Morelos, Mexico
Andrés C.:
ARC Centre of Excellence in Plant Energy Biology, Molecular and Chemical Sciences Building (M316), University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia
Unitede Rech./Genomique Veg., Inst. Natl. de la Rech. Agronom. Ctr. Natl. de la Rech. Sci., Universited'Evry Val d'Essonne, CP 5708, 91057 Evry Cedex, France
Gutierrez-Nava, MD:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62271, Morelos, Mexico
Cantero A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62271, Morelos, Mexico
Delannoy E.:
ARC Centre of Excellence in Plant Energy Biology, Molecular and Chemical Sciences Building (M316), University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia
Jiménez L.F.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Microscopia Elect, Mexico City 04510, DF, Mexico
Lurin C.:
Unitede Rech./Genomique Veg., Inst. Natl. de la Rech. Agronom. Ctr. Natl. de la Rech. Sci., Universited'Evry Val d'Essonne, CP 5708, 91057 Evry Cedex, France
Small I.:
ARC Centre of Excellence in Plant Energy Biology, Molecular and Chemical Sciences Building (M316), University of Western Australia, 35 Stirling Highway, Perth, WA 6009, Australia
Unitede Rech./Genomique Veg., Inst. Natl. de la Rech. Agronom. Ctr. Natl. de la Rech. Sci., Universited'Evry Val d'Essonne, CP 5708, 91057 Evry Cedex, France
Leo P.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62271, Morelos, Mexico
All Open Access; Bronze
|