Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum
Por:
Rojas A., Feregrino A., Ibarra-Alvarado C., Aguilar M.B., Falcon A., Heimer De La, CE
Publicada:
1 ene 2008
Resumen:
The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC50 = 0.11 +/- 0.02 mu g protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 mu M), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 mu M) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly inhibited contractions evoked by histamine (0.5 mu M), particularly those from C. spurius and P. albida. These findings reveal the presence of antihistaminergic compounds not previously described in any conoidean venom. Finally,
Filiaciones:
Rojas A.:
Laboratory of Chemical and Pharmacological Research on Natural Products, School of Chemistry, Autonomus University of Querétaro, Santiago de Querétaro, Querétaro State, Mexico
Laboratorio de Investigación Química y Farmacológica de Productos Naturales, Facultad de Quimica, Centro Universitario, Col. Centro, C.P. 76010, Queretaro, Qro, Mexico
Feregrino A.:
Laboratory of Chemical and Pharmacological Research on Natural Products, School of Chemistry, Autonomus University of Querétaro, Santiago de Querétaro, Querétaro State, Mexico
Department of Cellular and Molecular Neurobiology, Institute of Neurobiology, National Autonomous University of Mexico, Santiago de Querétaro, Querétaro State, Mexico
Ibarra-Alvarado C.:
Laboratory of Chemical and Pharmacological Research on Natural Products, School of Chemistry, Autonomus University of Querétaro, Santiago de Querétaro, Querétaro State, Mexico
Aguilar M.B.:
Department of Cellular and Molecular Neurobiology, Institute of Neurobiology, National Autonomous University of Mexico, Santiago de Querétaro, Querétaro State, Mexico
Falcon A.:
Department of Cellular and Molecular Neurobiology, Institute of Neurobiology, National Autonomous University of Mexico, Santiago de Querétaro, Querétaro State, Mexico
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