Identification of nucleoli in the early branching protist Giardia duodenalis
Por:
Jiménez-García L.F., Zavala G., Chávez-Munguía B., Ramos-Godinez, MDP, López-Velázquez G., Segura-Valdez, MDL, Montanez, C, Hehl A.B., Argüello-García R., Ortega-Pierres G.
Publicada:
1 sep 2008
Resumen:
Giardia duodenalis has been described as 'anucleolated'. In this work we analysed the subcellular distribution of several nucleolar markers in Giardia nuclei using silver and immunostaining techniques for electron and confocal laser microscopy as well as expression of epitope-tagged proteins in transgenic trophozoites. We identified anteronuclear fibrogranular structures corresponding to nucleolar organising regions with recruited ribonucleoprotein complexes, rRNA and epitope-tagged fibrillarin and rRNA-pseudouridine synthase (CBF5). Recombinant fibrillarin and CBF5 were targeted to this subcompartment. This study demonstrates the presence of nucleoli in G. duodenalis and provides a model to analyse minimal requirements for nucleolar assembly and maintenance in eukaryotic cells. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Microscopia, Inst Biotecnol, Cuernavaca 62191, Morelos, Mexico
Departamento de Patología Experimental, Centro de Investigación, Estudios Avanzados-IPN, Mexico City, Mexico
Laboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, Mexico City, Mexico
Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland
Departamento de Genética y Biología Molecular, Centro de Investigación, Estudios Avanzados-IPN, Mexico City, Mexico
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