A study of the process of apoptosis in animals infected with the contagious ecthyma virus
Por:
Garrido-Farina, GI, Cornejo-Cortes, MA, Martinez-Rodriguez, A, Reyes-Esparza, J, Alba-Hurtado, F, Tortora-Perez, J
Publicada:
25 may 2008
Resumen:
Contagious ecthyma virus (CEV) is a disease caused by a parapoxvirus, also is a potent genetic carrier with the capacity for regulating apoptosis in the cells of infected skin, a mechanism that serves for evading the immune response of the host. It has been suggested that the virus may remain in the skin and be able to cause repeated infections in the same flock. The effect of infection as well as the presence of contagious ecthyma virus was evaluated in terms of lesions and apoptosis in the skin of animals, infected both naturally and experimentally. Samples used were obtained from a naturally infected sheep, 5 goats inoculated with CEV and a negative control. Samples obtained were longitudinally sectioned and processed using photon and electron microscopy, and embedded in paraffin and araldite. Samples embedded in paraffin were sectioned in 5 mu m of thickness and dyed with orange eosin-hematoxilin G and Gomori's trichrom stain, apoptosis was demonstrated by the TUNEL assay, the vira
Filiaciones:
Garrido-Farina, GI:
FES Cuautitlan Univ Nacl Autonoma Mexico, Dept Biol Sci, Morphol Sci Sect, Support Lab Histol & Biol, Mexico City, DF, Mexico
Martinez-Rodriguez, A:
FES Cuautitlan Univ Nacl Autonoma Mexico, Dept Biol Sci, Multidisciplinary Unit Res Anim Hlth, Mexico City, DF, Mexico
Reyes-Esparza, J:
Autonomous Univ, Fac Pharm, Cytometry Unit, Mexico City, DF, Mexico
Alba-Hurtado, F:
FES Cuautitlan Univ Nacl Autonoma Mexico, Dept Biol Sci, Multidisciplinary Unit Res Anim Hlth, Mexico City, DF, Mexico
Tortora-Perez, J:
FES Cuautitlan Univ Nacl Autonoma Mexico, Dept Biol Sci, Multidisciplinary Unit Res Anim Hlth, Mexico City, DF, Mexico
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