Signaling the signal, cyclic AMP-dependent protein kinase inhibition by insulin-formed H2O2 and reactivation by thioredoxin
Por:
de Pina, MZ, Vazquez-Meza, H, Pardo, JP, Rendon, JL, Villalobos-Molina, R, Riveros-Rosas, H, Pina, E
Publicada:
2 may 2008
Resumen:
Catecholamines in adipose tissue promote lipolysis via cAMP, whereas insulin stimulates lipogenesis. Here we show that H2O2 generated by insulin in rat adipocytes impaired cAMP-mediated amplification cascade of lipolysis. These micromolar concentrations of H2O 2 added before cAMP suppressed cAMP activation of type IIßcyclic AMP-dependent protein kinase (PKA) holoenzyme, prevented hormone-sensitive lipase translocation from cytosol to storage droplets, and inhibited lipolysis. Similarly, H2O2 impaired activation of type IIa PKA holoenzyme from bovine heart and from that reconstituted with regulatory IIa and catalytic a subunits. H2O2 was ineffective (a) if these PKA holoenzymes were preincubated with cAMP, (b) if added to the catalytic a subunit, which is active independently of cAMP activation, and (c) if the catalytic a subunit was substituted by its C199A mutant in the reconstituted holoenzyme. H2O2 inhibition of PKA activation remained after H2O2 elimination by gel filtration but was reverted with dithiothreitol or with thioredoxin reductase plus thioredoxin. Electrophoresis of holoenzyme in SDS gels showed separation of catalytic and regulatory subunits after cAMP incubation but a single band after H2O2 incubation. These data strongly suggest that H2O2 promotes the formation of an intersubunit disulfide bond, impairing cAMP dependent PKA activation. Phylogenetic analysis showed that Cys-97 is conserved only in type II regulatory subunits and not in type I regulatory subunits; hence, the redox regulation mechanism described is restricted to type II PKA-expressing tissues. In conclusion, phylogenetic analysis results, selective chemical behavior, and the privileged position in holoenzyme lead us to suggest that Cys-97 in regulatory IIa or IIß subunits is the residue forming the disulfide bond with Cys-199 in the PKA catalytic a subunit. A new molecular point for cross-talk among heterologous signal transduction pathways is demonstrated. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 04510, DF, Mexico
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