Regulation of the cellular localization and function of human transient receptor potential channel 1 by other members of the TRPC family
Por:
Alfonso S., Benito O., Alicia S., Angélica Z., Patricia G., Diana K., Luis V.
Publicada:
1 abr 2008
Resumen:
Members of the Canonical Transient Receptor Potential (TRPQ family of ionic channels are able to form homo- and heterotetrameric channels. Depending on the study, TRPC1 has been detected on both the surface and inside the cell, probably in the endoplasmic reticulum (ER). Likewise, TRPC I has been described both as a store-operated channel and as one unable to function when forming a homotetramer. It is possible that the apparent differences in the expression and function of TRPC1 are due to its association with other proteins, possibly from the same TRPC family. In the present study we used confocal microscopy and a fluorescently tagged TRPC I to examine the localization of this protein when co-expressed with other members of the TRPC family. Whole-cell and single channel electrophysiological recordings were conducted to study the function of TRPC I expressed alone or co-expressed with other members of the TRPC family. A FRET-based calcium sensor fused to TRPCI was used to assess the f
Filiaciones:
Alfonso S.:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Cell Biol, Mexico City 04510, DF, Mexico
Benito O.:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Cell Biol, Mexico City 04510, DF, Mexico
Alicia S.:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Cell Biol, Mexico City 04510, DF, Mexico
Angélica Z.:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Cell Biol, Mexico City 04510, DF, Mexico
Patricia G.:
Rammelkamp Center for Education and Research, Case Western Reserve University, MetroHealth Campus and Dep. Neurosc., 2500 MetroHealth Dr., Cleveland, OH 44109, United States
Diana K.:
Rammelkamp Center for Education and Research, Case Western Reserve University, MetroHealth Campus and Dep. Neurosc., 2500 MetroHealth Dr., Cleveland, OH 44109, United States
Luis V.:
Univ Nacl Autonoma Mexico, Inst Fis, Dept Cell Biol, Mexico City 04510, DF, Mexico
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