The polypeptides COX2A and COX2B are essential components of the mitochondrial cytochrome c oxidase of Toxoplasma gondii
Por:
Morales-Sainz, L, Escobar-Ramirez, A, Cruz-Torres, V, Reyes-Prieto, A, Vazquez-Acevedo, M, Lara-Martinez, R, Jimenez-Garcia, LF, Gonzalez-Halphen, D
Publicada:
1 feb 2008
Resumen:
Two genes encoding cytochrome c oxidase subunits, Cox2a and Cox2b, are present in the nuclear genomes of apicomplexan parasites and show sequence similarity to corresponding genes in chlorophycean algae. We explored the presence of COX2A and COX2B subunits in the cytochrome c oxidase of Toxoplasma gondii. Antibodies were raised against a synthetic peptide containing a 14-residue fragment of the COX2A polypeptide and against a hexa-histidine-tagged recombinant COX2B protein. Two distinct immunochemical stainings localized the COX2A and COX2B proteins in the parasite's mitochondria. A mitochondria-enriched fraction exhibited cyanide-sensitive oxygen uptake in the presence of succinate. T gondii mitochondria were solubilized and subjected to Blue Native Electrophoresis followed by second dimension electrophoresis. Selected protein spots from the 2D gels were subjected to mass spectrometry analysis and polypeptides of mitochondrial complexes 111, IV and V were identified. Subunits COX2A an
Filiaciones:
Morales-Sainz, L:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Escobar-Ramirez, A:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Cruz-Torres, V:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Reyes-Prieto, A:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Vazquez-Acevedo, M:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
Lara-Martinez, R:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, DF, Mexico
Jimenez-Garcia, LF:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, DF, Mexico
Gonzalez-Halphen, D:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
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