Identification, molecular characterisation and differential expression of caveolin-1 in Trichinella spiralis maturing oocytes and embryos
Por:
Hernández-Bello R., Bermúdez-Cruz R.M., Fonseca-Liñán R., García-Reyna P., Le Guerhier F., Boireau P., Ortega-Pierres G.
Publicada:
1 feb 2008
Resumen:
Caveolins are integral membrane proteins implicated in cholesterol homeostasis and transport, endocytosis mechanisms and regulation of signal transduction in differentiated cells. In this work a caveolin-1 gene from the nematode Trichinella spiralis (Ts-cav-1) was cloned and identified as an adult-specific antigen. For this, a cDNA library of T spiralis 3-day-old adult worms was screened using a stage-specific cl)NA-labelled probe. One positive clone contained a cDNA insert of 1427-bp and a full-length open reading frame (ORF) of 687-bp, which encodes for a 229 amino acid polypeptide with a theoretical molecular weight of 26 kDa. BLAST and FASTA searches revealed a 36W% and 57% identity with Caenorhabditis elegans caveolin-1, respectively. Confocal laser microscopy analysis using antibodies generated against Ts-CAV-1 protein and cross-sections of adult parasites showed that Ts-CAV-1 gradually accumulates on the surface of Trichinella oocytes and embryos, reaching a maximum at 3 days p.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cautitlan, Mexico City 04510, DF, Mexico
Department of Genetics and Molecular Biology, Centro de Investigación y Estudios Avanzados, IPN, 07360 México City, Mexico
AFSSA-INRA-ENVA-UPVM, UMR BIPAR, Maisons-Alfort, France
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