Immunoglobulin Concentrations in Plasma and Saliva During the Neonatal Period
Por:
Pineda-Martínez S., Hernández-Islas J.L., Escobedo-Torres M.P., Paredes-Alonzo I.E., López-Candiani C., Correa D., Vela-Amieva M.
Publicada:
1 jun 2016
Categoría:
Pediatrics, Perinatology and Child Health
Resumen:
Background: Screening for infectious diseases in newborns using
immunoglobulin (Ig)A-, IgM-, and IgE-specific antibodies is expensive
and impractical. To determine if total levels of these Igs can be used
for screening purposes, thus simplifying the process, their basic levels
in the 1st month of extrauterine life need to be determined.
Additionally, the ability to simplify screening by using saliva also
needs to be determined. The aim of this study was to determine IgA, IgM,
and IgE concentrations in plasma and saliva in newborns, correlation
between the samples, and relationship between Ig levels and newborn age.
Methods: We enrolled 53 apparently healthy newborns, paired samples of
plasma and saliva were collected, and total IgA, IgM, and IgE
concentrations determined by capture enzyme linked immunosorbent assay.
The correlation between plasma and saliva values was calculated by
Spearman's rank correlation coefficient and the IgA, IgM, and IgE
distributions were analyzed by the Shapiro-Wilk test. We also determined
the level of each Ig concentration according to age.
Results: IgA and IgM levels in plasma and IgA levels in saliva increased
significantly during 1st month of life, especially in the 2nd week and
3rd week, with a good correlation of IgA between plasma and saliva. IgE
levels in both plasma and saliva and IgM levels in saliva were very low
or absent.
Conclusion: These results suggest that Igs in saliva could be good
biomarkers for newborn screening programs during the 1st week of life.
This study established reference values for Igs according to age in the
neonatal period. Copyright (C) 2015, Taiwan Pediatric Association.
Published by Elsevier Taiwan LLC. This is an open access article under
the CC BY-NC-ND license.
Filiaciones:
Pineda-Martínez S.:
Laboratorio de Errores Innatos Del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Avenida Insurgentes Sur 3700-C, Colonia-Insurgentes Cuicuilco, México, D.F., C.P. 04530, Mexico
Universidad Nacional Autónoma de México, Programa de Maestría y Doctorado en Ciencias Médicas, Odontológicas y de la Salud, México, D.F., Mexico
Hernández-Islas J.L.:
Laboratorio de Errores Innatos Del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Avenida Insurgentes Sur 3700-C, Colonia-Insurgentes Cuicuilco, México, D.F., C.P. 04530, Mexico
Escobedo-Torres M.P.:
Hospital General Dr. Manuel Gea González, México, D.F., Mexico
Paredes-Alonzo I.E.:
Hospital General Dr. Manuel Gea González, México, D.F., Mexico
López-Candiani C.:
Laboratorio de Errores Innatos Del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Avenida Insurgentes Sur 3700-C, Colonia-Insurgentes Cuicuilco, México, D.F., C.P. 04530, Mexico
Correa D.:
Laboratorio de Errores Innatos Del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Avenida Insurgentes Sur 3700-C, Colonia-Insurgentes Cuicuilco, México, D.F., C.P. 04530, Mexico
Vela-Amieva M.:
Laboratorio de Errores Innatos Del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Avenida Insurgentes Sur 3700-C, Colonia-Insurgentes Cuicuilco, México, D.F., C.P. 04530, Mexico
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