An integrative taxonomic study reveals a new species of Tylodelphys Diesing, 1950 (Digenea: Diplostomidae) in central and northern Mexico
Por:
García-Varela M., Sereno-Uribe A.L., Pinacho-Pinacho C.D., Hernández-Cruz E., Pérez-Ponce De León G.
Publicada:
1 nov 2016
Resumen:
Tylodelphys aztecae n. sp. (Digenea: Diplostomidae) is described from
adult specimens obtained from the intestine of the pied-billed grebe
(Podilymbus podiceps) and the metacercariae found in the body cavity of
freshwater fishes of the families Goodeidae and Cyprinidae in eight
localities across central and northern Mexico. The new species is mainly
distinguished from the other four described species of Tylodelphys from
the Americas (T. adulta, T. americana, T. elongata and T. brevis) by
having a forebody slightly concave, a larger ventral sucker, two larger
pseudosuckers and by having between 2 and 7 eggs in the uterus. Partial
DNA sequences of the mitochondrial gene cytochrome c oxidase subunit I
(cox1), and the internal transcribed spacers (ITS1 + 5.8S + ITS2) of the
ribosomal DNA, were generated for both developmental stages and compared
with available sequences in GenBank of other congeners. The genetic
divergence estimated among Tylodelphys aztecae n. sp. and other
congeneric species varied from 12 to 15% for cox1, and from 3 to 11%
for ITS. In contrast, the genetic divergence among metacercariae and
adults of the new species was very low, ranging between 0 and 1% for
cox1 and between 0 and 0.3% for ITS. Phylogenetic analyses inferred
with both molecular markers using maximum likelihood and Bayesian
inference placed the adults and their metacercariae in a single clade,
confirming that both stages are conspecific. The morphological evidence
and the genetic divergence, in combination with the reciprocal monophyly
in both phylogenetic trees, support the hypothesis that the diplostomids
found in the intestines of the pied-billed grebe bird and the body
cavity from goodeid and cyprinid fishes in central and northern Mexico
represent a new species.
Filiaciones:
García-Varela M.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Distrito Federal, C.P. 04510, Mexico
Sereno-Uribe A.L.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Distrito Federal, C.P. 04510, Mexico
Pinacho-Pinacho C.D.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Distrito Federal, C.P. 04510, Mexico
Hernández-Cruz E.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Distrito Federal, C.P. 04510, Mexico
Pérez-Ponce De León G.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Zool, Ave Univ 3000,Ciudad Univ, Mexico City 04510, DF, Mexico
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Distrito Federal, C.P. 04510, Mexico
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