Exopolysaccharides from Bifidobacterium longum subsp. infantis and Bifidobacterium adolescentis modulate Toll-like receptor signaling


Por: Akkerman R., Oerlemans M.M.P., Ferrari M., Fernández-Lainez C., Walvoort M.T.C., de Vos P.

Publicada: 1 ene 2025
Resumen:
Background: Exopolysaccharides (EPS) from probiotic bacteria like bifidobacteria, have gained considerable attention for the beneficial effects they exert in the gastrointestinal environment. Here, we investigated whether EPS isolated from Bifidobacterium longum subsp. infantis and Bifidobacterium adolescentis can interact with Toll-like receptors (TLRs) in a structure-dependent way and subsequently we investigated whether they influence cytokine-production in dendritic cells (DCs). Results: EPS from both B. infantis and B. adolescentis were found to be structurally different and were able to inhibit signaling of TLR2 and TLR4 in an EPS-type dependent fashion. EPS from B. infantis was shown to have stronger inhibitory effects on TLR2/1, whereas EPS from B. adolescentis showed stronger effects for TLR2/6 and TLR4. Incubation of DCs with EPS alone had no effect, however stimulation of DCs with spend-medium of epithelial cells incubated with EPS reduced production of the cytokines MCP-1/CCL2 and TNFa. Conclusion: Here we show that EPS from B. infantis and B. adolescentis have structure-dependent immunomodulatory effects, indicating that EPS might be important effector molecules responsible for the health benefits of bifidobacteria. © 2024 The Authors

Filiaciones:
Akkerman R.:
 Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, Netherlands

Oerlemans M.M.P.:
 Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, Netherlands

Ferrari M.:
 Department of Chemical Biology, Stratingh Institute for Chemistry, University of Groningen, Groningen, Netherlands

Fernández-Lainez C.:
 Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, Netherlands

 Laboratorio de Errores Innatos del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Ciudad de México, Mexico

Walvoort M.T.C.:
 Department of Chemical Biology, Stratingh Institute for Chemistry, University of Groningen, Groningen, Netherlands

de Vos P.:
 Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, Netherlands
ISSN: 01448617
Editorial
ELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND, Reino Unido
Tipo de documento: Article
Volumen: 349 Número:
Páginas:
WOS Id: 001363809200001
ID de PubMed: 39638524
imagen All Open Access; Hybrid Gold Open Access