Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering
Por:
Hidalgo-Vicelis J.L., Rivera-Contreras A.R., Hernández-Téllez B., Piñón-Zárate G., Jarquín-Yáñez K., Fiordelisio-Coll T., Saniger-Blesa J.M., González-Gómez G.H., Falcón-Neri M.A., Canales-Martínez M.M., Castell-Rodríguez A.E.
Publicada:
1 ene 2025
Resumen:
Currently, there are no therapies that prevent the negative myocardial remodeling process that occurs after a heart attack. Injectable hydrogels are a treatment option because they may replace the damaged extracellular matrix and, in addition, can be administered minimally invasively. Reactive oxygen species generated by ischemia-reperfusion damage can limit the therapeutic efficacy of injectable hydrogels. In order to overcome this limitation, grape seed proanthocyanidins were incorporated as antioxidant compounds into a thermosensitive myocardial extracellular matrix hydrogel in this study. For the fabrication of the hydrogel, the extracellular matrix obtained by decellularization of porcine myocardium was solubilized through enzymatic digestion, and the proanthocyanidins were incorporated. After exposing this extracellular matrix solution to 37 °C, it self-assembled into a hydrogel with a porous structure. According to the physicochemical and biological evaluation, the coupling of proanthocyanidins in the hydrogel has a positive effect on the antioxidant capacity, gelation kinetics, in vitro degradation, and cardiomyocyte viability, indicating that the hydrogel coupled with this type of antioxidants represents a promising alternative for potential application in post-infarction myocardial regeneration. Furthermore, this study proposes the best concentrations of proanthocyanidins that resulted in the hydrogels for future studies in cardiac tissue engineering. © 2025 by the authors.
Filiaciones:
Hidalgo-Vicelis J.L.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
Rivera-Contreras A.R.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
Hernández-Téllez B.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
Piñón-Zárate G.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
Jarquín-Yáñez K.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
Fiordelisio-Coll T.:
Laboratory of Comparative Neuroendocrinology, Department of Biology, Faculty of Sciences, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000Ciudad de México, Coyoacán, 04510, Mexico
Saniger-Blesa J.M.:
Group of Nanostructured Supports, Department of Micro and Nanotechnologies, Institute of Applied Sciences and Technology, National Autonomous University of Mexico, Av. Universidad 3000, Copilco UniversidadCiudad de México, Coyoacán, 04510, Mexico
González-Gómez G.H.:
Laboratory of Functional Biophysics, Department of Physics, Faculty of Sciences, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000Ciudad de México, Coyoacán, 04510, Mexico
Falcón-Neri M.A.:
Laboratory of Functional Biophysics, Department of Physics, Faculty of Sciences, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000Ciudad de México, Coyoacán, 04510, Mexico
Canales-Martínez M.M.:
Laboratory of Pharmacognosy, Unit of Biotechnology and Prototypes, Faculty of Higher Studies Iztacala, National Autonomous University of Mexico, Avenida de los Barrios 1, Los Reyes IztacalaEstado de México, Tlalnepantla de Baz, 54090, Mexico
Castell-Rodríguez A.E.:
Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Copilco Universidad, Av. Universidad 3000 Ciudad de México, Coyoacán, 04510, Mexico
gold, All Open Access; Gold Open Access
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