A core genome MLST scheme for Borrelia burgdorferi sensu lato improves insights into the evolutionary history of the species complex
Por:
Hepner S., Jolley K.A., Castillo-Ramirez S., Mourkas E., Dangel A., Wieser A., Hübner J., Sing A., Fingerle V., Margos G.
Publicada:
1 ene 2025
Ahead of Print:
1 ene 2025
Resumen:
Multi-locus sequence typing (MLST) based on eight genes has become the method of choice for Borrelia typing and is extensively used for population studies. Whole-genome sequencing enables studies to scale up to genomic levels but necessitates extended schemes. We have developed a 639-loci core genome MLST (cgMLST) scheme for Borrelia burgdorferi sensu lato (s.l.) that enables unambiguous genotyping and improves the robustness of phylogenies and lineage resolution within species. Notably, all inner nodes of the cgMLST phylogenies had consistently high statistical support. Analyses of the genetically homogeneous European B. bavariensis population support the notion that cgMLST provides high discriminatory power even for closely related isolates. While isolates differed maximally in one MLST locus, there were up to 179 cgMLST loci differences. Thus, the developed cgMLST scheme for B. burgdorferi s.l. resolves lineages at a finer resolution than MLST and improves insights into the evolutionary history of the species complex. © 2024 The Authors
Filiaciones:
Hepner S.:
German National Reference Centre for Borrelia, Oberschleissheim, Germany
Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
Jolley K.A.:
Department of Biology, University of Oxford, Oxford, United Kingdom
Castillo-Ramirez S.:
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Mexico City, Mexico
Mourkas E.:
Zoonosis Science Centre, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
Dangel A.:
Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
Wieser A.:
Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Munich, Germany
Division of Infectious Diseases and Tropical Medicine, LMU University Hospital, LMU Munich, Munich, Germany
German Center for Infection Research (DZIF), Partner Site Munich, Munich, Germany
Immunology, Infectious Disease and Pandemic Research (IIP), Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Munich, Germany
Hübner J.:
Dr. von Hauner Children's Hospital, LMU Munich, Munich, Germany
Sing A.:
German National Reference Centre for Borrelia, Oberschleissheim, Germany
Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
Fingerle V.:
German National Reference Centre for Borrelia, Oberschleissheim, Germany
Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
Margos G.:
German National Reference Centre for Borrelia, Oberschleissheim, Germany
Bavarian Health and Food Safety Authority, Oberschleissheim, Germany
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