Experimental Lung Transplantation Related With HIF-1, VEGF, ROS. Assessment of HIF-1a, VEGF, and Reactive Oxygen Species After Competitive Blockade of Chetomin for Lung Transplantation in Rats
Por:
Bravo-Reyna C.C., Zentella A., Venturagallegos J.L., Torres-Villalobos G., Miranda-Galván V., Alanis-Mendizabal J., Escobar-Valderrama J.M., Nava C., Díaz-Martínez N.E., Bliskunova T., Morales-De Los Santos V.C.
Publicada:
1 ene 2024
Resumen:
Primary graft failure occurs 15 to 30 % of the time after transplantation. Although there have been improvements in preserving the lungs in good condition, there have not been studies on the regulation of transcription factors. Methods: We carried out an experimental study involving lung transplantation to indirectly evaluate reactive oxygen species (ROS) production and VEGF expression by competitive blockade of HIF-1a with chetomin. There were 5 groups: Group-1: Lung blocks were perfused with 0.9 % SSF, immediately harvested, and preserved.Group-2 (I-T): Immediate transplantation and then reperfusion for 1 h. Group-3 (I-R): Lung blocks were harvested and preserved in LPD solution for 6 h and reperfused for 1 h. Group-4 (DMSO): Lung blocks were treated for 4 h with DMSO, preserved for 6 h and transplanted to a receptor treated with DMSO.Group-5 (chetomin): Lung blocks were treated for 4 h with chetomin, preserved for 6 h and transplanted to a receptor treated with chetomin. ROS, mRNA, and protein levels of HIF-1a and EG-VEGF were determined. Results: The DMSO and chetomin groups had significantly lower ROS levels. Compared with those in the I-R group, the chetomin group exhibited the lowest level of HIF-1a. Conclusions: Addition of chetomin to the donor and the receptor results in a significant reduction in HIF-1A, VEGF and ROS. © (2024), (Czech Academy of Sciences). All rights reserved.
Filiaciones:
Bravo-Reyna C.C.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Zentella A.:
Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico, Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Mexico
Venturagallegos J.L.:
Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico, Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Mexico
Torres-Villalobos G.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Miranda-Galván V.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Alanis-Mendizabal J.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Escobar-Valderrama J.M.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Nava C.:
Departamento de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara, Mexico
Díaz-Martínez N.E.:
Departamento de Bioquímica y Nutrición Animal, Facultad de Medicina Veterinaria y Zootecnia, UNAM, CDMX, Mexico
Bliskunova T.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
Morales-De Los Santos V.C.:
Departamento de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, CDMX, Mexico
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