Fc-gamma receptor expression and cytokine responses to intravenous human immunoglobulin in whole blood from non-pregnant and pregnant women and newborns
Por:
Vázquez-Rodríguez S., Arriaga-Pizano L.A., Mancilla-Herrera I., Prieto-Chávez J., Arizmendi-Villanueva R., Torres-Rosas R., Flisser A., García-Latorre E., Cérbulo-Vázquez A.
Publicada:
1 ene 2024
Ahead of Print:
1 ene 2024
Resumen:
BACKGROUND: Intravenous immunoglobulin (IVIG) can suppress the inflammatory response in adults, but its role in pregnant women and newborns is poorly studied. While the adult immune system is considered mature, it is immature in neonates and suppressed in pregnancy. Since the immune response differs in these 3 groups, the use of IVIG could differentially modulate the immune response. OBJECTIVES: We aimed to explore the effect of IVIG on myeloid blood cells from non-pregnant women, pregnant women and newborns. MATERIAL AND METHODS: Whole blood from healthy donors was incubated with lipopolysaccharide (LPS) and/or IVIG. After 0 h, 24 h and 48 h of culture, Fc-gamma receptor (CD16, CD32 and CD64) expression, monocyte and neutrophil bacterial phagocytosis, and cytokine and chemokine concentrations were determined in the supernatant. RESULTS: The baseline expression of monocyte CD16 was higher in newborns than in adult women, but the expression of CD32 and CD64 was similar between groups. Furthermore, LPS and IVIG stimulation, together or separately, did not change Fc-gamma receptor expression in monocytes or neutrophils and did not modify their phagocytosis capacity. On the other hand, IVIG did not downregulate the proinflammatory cytokine response induced by LPS in any group. Interestingly, IVIG induced a strong interleukin 8 (IL-8) response in neonates but not in non-pregnant or pregnant women. CONCLUSIONS: Our results show that IVIG did not induce changes in Fc-gamma receptor expression, phagocytic ability, or the cytokine response to LPS in blood cells from neonates, non-pregnant or pregnant women. However, IVIG induced a strong IL-8 response in neonates that could improve immunity.
Filiaciones:
Vázquez-Rodríguez S.:
Department of Physiology and Cellular Development, National Institute of Perinatology (INPer), Mexico City, Mexico
Department of Immunology, National School of Biomedical Sciences, Mexico City, Mexico
Arriaga-Pizano L.A.:
School of Dentistry, Benito Juárez Autonomous University of Oaxaca, Mexico
Medical Research Unit in Immunochemistry, Specialty Hospital "Dr. Bernardo Sepúlveda Gutiérrez", Mexico City, Mexico
Mancilla-Herrera I.:
Department of Infectious Diseases and Immunology, National Institute of Perinatology (INPer), Mexico City, Mexico
Prieto-Chávez J.:
Department of Immunology, National School of Biomedical Sciences, Mexico City, Mexico
Medical Research Unit in Immunochemistry, Specialty Hospital "Dr. Bernardo Sepúlveda Gutiérrez", Mexico City, Mexico
Arizmendi-Villanueva R.:
Women's Hospital, Secretariat of Health, Mexico City, Mexico
Torres-Rosas R.:
School of Dentistry, Benito Juárez Autonomous University of Oaxaca, Mexico
Flisser A.:
Faculty of Medicine, Department of Microbiology and Parasitology, National Autonomous University of Mexico (UNAM), Mexico City, Mexico
García-Latorre E.:
Department of Immunology, National School of Biomedical Sciences, Mexico City, Mexico
Cérbulo-Vázquez A.:
Genomic Medicine Service, General Hospital of Mexico "Dr. Eduardo Liceaga", Mexico City, Mexico
gold, All Open Access; Gold Open Access
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