Amorphous TiO2 nano-coating on stainless steel to improve its biological response
Por:
Garcia-Perez, VI, Hotchkiss, KM, Silva-Bermudez, P, Hernández, MM, Prado-Prone, G, Olivares-Navarrete, R, Rodil, SE, Almaguer-Flores, A
Publicada:
1 sep 2024
Resumen:
This study delves into the potential of amorphous titanium oxide (aTiO(2)) nano-coating to enhance various critical aspects of non-Ti-based metallic orthopedic implants. These implants, such as medical-grade stainless steel (SS), are widely used for orthopedic devices that demand high strength and durability. The aTiO(2) nano-coating, deposited via magnetron sputtering, is a unique attempt to improve the osteogenesis, the inflammatory response, and to reduce bacterial colonization on SS substrates. The study characterized the nanocoated surfaces (SS-a TiO2) in topography, roughness, wettability, and chemical composition. Comparative samples included uncoated SS and sandblasted/acid-etched Ti substrates (Ti). The biological effects were assessed using human mesenchymal stem cells (MSCs) and primary murine macrophages. Bacterial tests were carried out with two aerobic pathogens (S. aureus and S. epidermidis) and an anaerobic bacterial consortium representing an oral dental biofilm. Results from this study provide strong evidence of the positive effects of the aTiO(2) nano-coating on SS surfaces. The coating enhanced MSC osteoblastic differentiation and exhibited a response similar to that observed on Ti surfaces. Macrophages cultured on aTiO(2) nano-coating and Ti surfaces showed comparable anti-inflammatory phenotypes. Most significantly, a reduction in bacterial colonization across tested species was observed compared to uncoated SS substrates, further supporting the potential of aTiO(2) nano-coating in biomedical applications. The findings underscore the potential of magnetron-sputtering deposition of aTiO(2) nano-coating on non-Ti metallic surfaces such as medical-grade SS as a viable strategy to enhance osteoinductive factors and decrease pathogenic bacterial adhesion. This could significantly improve the performance of metallic-based biomedical devices beyond titanium.
Filiaciones:
Garcia-Perez, VI:
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Lab Biointerfases, Circuito Exterior S-N, Ciudad De Mexico 04510, Cdmx, Mexico
Hotchkiss, KM:
Virginia Univ, Coll Engn, Dept Biomed Engn Commonwealth, Richmond, VA 23284 USA
Silva-Bermudez, P:
Inst Nacl Rehabil Luis Guillermo Ibarra Ibarra, Unidad Ingn Tejidos Terapia Celular & Med Regenera, Ciudad De Mexico 14389, Mexico
Hernández, MM:
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Lab Biointerfases, Circuito Exterior S-N, Ciudad De Mexico 04510, Cdmx, Mexico
Prado-Prone, G:
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Lab Biointerfases, Circuito Exterior S-N, Ciudad De Mexico 04510, Cdmx, Mexico
Olivares-Navarrete, R:
Virginia Univ, Coll Engn, Dept Biomed Engn Commonwealth, Richmond, VA 23284 USA
Rodil, SE:
Univ Nacl Autonoma Mexico, Inst Invest Mat, Circuito Exterior S-N,Ciudad Univ, Ciudad De Mexico 04510, Cdmx, Mexico
Almaguer-Flores, A:
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrad & Invest, Lab Biointerfases, Circuito Exterior S-N, Ciudad De Mexico 04510, Cdmx, Mexico
Green Published, hybrid, All Open Access; Hybrid Gold Open Access
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