Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-human Acinetobacter baumannii
Por:
Sykes E.M.E., Mateo-Estrada V., Engelberg R., Muzaleva A., Zhanel G., Dettman J., Chapados J., Gerdis S., Akineden Ö., Khan I.U.H., Castillo-Ramírez S., Kumar A.
Publicada:
1 ene 2024
Ahead of Print:
1 mar 2024
Resumen:
Acinetobacter baumannii is a Gram-negative, opportunistic pathogen that causes infections in the immunocompromised. With a high incidence of muti-drug resistance, carbapenem-resistant A. baumannii is designated as a priority 1 pathogen by the WHO. The current literature has expertly characterized clinical isolates of A. baumannii. As the challenge of these infections has recently been classifiedas a One Health issue, we set out to explore the diversity of isolates from human and non-clinical sources, such as agricultural surface water, urban streams, various effluentsfrom wastewater treatment plants, and food (tank milk); and, importantly, these isolates came from a wide geographic distribution. Phylogenomic analysis considering almost 200 isolates showed that our diverse set is well-differentiatedfrom the main international clones of A. baumannii. We discovered novel sequence types in both hospital and non-clinical settings and fivestrains that overexpress the resistance-nodulation-division effluxpump adeIJK without changes in susceptibility reflectedby this overexpression. Furthermore, we detected a bla ADC-79 in a non-human isolate despite its sensitivity to all antibiotics. There was no significantdifferentiationbetween the virulence profilesof clinical and non-clinical isolates in the Galleria mellonella insect model of virulence, suggesting that virulence is neither dependent on geographic origin nor isolation source. The detection of antibiotic resistance and virulence genes in non-human strains suggests that these isolates may act as a genetic reservoir for clinical strains. This endorses the notion that in order to combat multi-drug-resistant infection caused by A. baumannii, a One Health approach is required, and a deeper understanding of non-clinical strains must be achieved. © 2024 Sykes et al.
Filiaciones:
Sykes E.M.E.:
Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada
Mateo-Estrada V.:
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Engelberg R.:
Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada
Muzaleva A.:
Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada
Zhanel G.:
Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada
Dettman J.:
Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada
Chapados J.:
Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada
Gerdis S.:
Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada
Akineden Ö.:
Dairy Sciences, Institute of Veterinary Food Science Justus-Liebig, University of Giessen, Giessen, Germany
Khan I.U.H.:
Ottawa Research and Development Centre (ORDC), Agriculture and Agri-Food Canada, Ottawa, ON, Canada
Castillo-Ramírez S.:
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Kumar A.:
Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada
gold, Green Submitted
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