Cytotoxic and Antitumoral Effects of Methanolic Extracts of Avocado Fruit Mesocarp in Colorectal Cancer Cell Line HT29
Por:
Gutierrez-Silerio G.Y., Garcia-Solis P., Yahia E.M., Núñez-Ríos J.D., Vázquez-Cuevas F., Rodriguez-Salinas P.A., Mendoza-Zuñiga R., Kuri-García A.
Publicada:
1 ene 2024
Ahead of Print:
1 feb 2024
Resumen:
Colorectal cancer is a widespread neoplasia with high ratios of chemoresistance. Phytochemicals in plant-based extracts could be useful to treat colorectal cancer, and/or reduce chemoresistance. Methanolic extract of avocado mesocarp (MEAM) has demonstrated antitumoral properties, depending on the fruit ripening stage (RS). The aim of this study was to analyze the effects of methanolic extracts of “Hass” avocado fruit at different RS on cytotoxicity, antioxidative, anti-inflammatory, anti-invasive, cell cycle, and epithelial-mesenchymal transition inhibition in colorectal adenocarcinoma cell line HT29. The MEAM showed an increasing concentration of total phenolic compounds as the RS progressed, which was correlated with antioxidant capacity measured by the Ferric Reducing Antioxidant Power assay but not with the 2.2-diphenyl-1-picrylhydrazyl assay. The specific phenolic compounds of MEAM were determined by high-performance liquid chromatography, and it was found that concentrations of epicatechin decreased while concentrations of chlorogenic acid increased as the RS progressed. The HT29 cell line was treated with MEAM for 48 h, and all MEAM had a cytotoxic effect, reported by MTT assay, nevertheless, the strongest effect was associated with the presence of chlorogenic acid. MEAM induced apoptosis and cell cycle arrest in phase G0/G1, reported by flow cytometry. Moreover, MEAM inhibited cell migration evidenced by the wound healing assay. On the other hand, MEAM significantly reduced expression of mRNA of tumor necrosis factor-alpha and cyclooxygenase 2. These effects comprise important inhibition of some hallmarks of cancer. This, in turn, may provide interesting guidelines for developing antitumoral intervention agents. Copyright 2024, Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition.
Filiaciones:
Gutierrez-Silerio G.Y.:
Endocrinology and Nutrition Laboratory, Center of Advanced Biomedical Research, School of Medicine, Autonomous University of Queretaro, Queretaro, Mexico
Garcia-Solis P.:
Endocrinology and Nutrition Laboratory, Center of Advanced Biomedical Research, School of Medicine, Autonomous University of Queretaro, Queretaro, Mexico
Yahia E.M.:
Phytochemicals and Nutrition Laboratory, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
Núñez-Ríos J.D.:
Cellular Physiology Laboratory, Department of Molecular and Cellular Neurobiology, Neurobiology Institute, National Autonomous Univeristy of Mexico, Queretaro, Mexico
Vázquez-Cuevas F.:
Cellular Physiology Laboratory, Department of Molecular and Cellular Neurobiology, Neurobiology Institute, National Autonomous Univeristy of Mexico, Queretaro, Mexico
Rodriguez-Salinas P.A.:
Phytochemicals and Nutrition Laboratory, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
Mendoza-Zuñiga R.:
Phytochemicals and Nutrition Laboratory, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
Kuri-García A.:
Phytochemicals and Nutrition Laboratory, School of Natural Sciences, Autonomous University of Queretaro, Queretaro, Mexico
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