Unraveling the diversity of Trypanosoma species from Central Mexico: Molecular confirmation on the presence of Trypanosoma dionisii and novel Neobat linages
Por:
Juárez-Gabriel J., Alegría-Sánchez D., Yáñez-Aguirre D., Grostieta E., Álvarez-Castillo L., Torres-Castro M., Aréchiga-Ceballos N., Moo-Llanes D.A., Alves F.M., Pérez-Brígido C.D., Aguilar-Tipacamú G., López González C.A., Becker I., Pech-Canché J.M., Colunga-Salas P., Sánchez-Montes S.
Publicada:
1 ene 2024
Ahead of Print:
1 ene 2024
Resumen:
Bats are one of the groups of mammals with the highest number of associated Trypanosoma taxa. There are 50 Trypanosoma species and genotypes infecting more than 75 species of bats across five continents. However, in Mexico, the inventory of species of the genus Trypanosoma associated with bats is limited to only two species (Trypanosoma vespertilionis and Trypanosoma cruzi) even though 140 species of bats inhabit this country. Specifically, 91 bat species have been recorded in the state of Veracruz, but records of trypanosomatids associated with this mammalian group are absent. Due to the complex Trypanosoma–bat relationship, the high diversity of bat species in Veracruz, as well as the lack of records of trypanosomatids associated with bats for this state, the aim of this work was to analyze the diversity of species of the genus Trypanosoma and their presence from a bat community in the central area of the state of Veracruz, Mexico. During the period of January to August 2022 in the Tequecholapa Environmental Management Unit where bats were collected using mist nets and blood samples were obtained from their thumbs. We extracted genetic material and amplified a fragment of 800 bp of the 18S ribosomal gene of the genus Trypanosoma by conventional PCR. The positive amplicons were sequenced, and phylogenetic reconstruction was performed to identify the parasite species. A total of 285 bats (149?, 136?) belonging to 13 species from 10 genera and a single family (Phyllostomidae) were collected. Twenty-three specimens from six species tested positive for the presence of Trypanosoma dionisii, Trypanosoma sp. Neobat 4, and a potential novelty species provisionally named as Trypanosoma sp. Neobat 6. The results of the present work increase the number of species of the genus Trypanosoma infecting bats in Mexico and in the Neotropical region. © 2023
Filiaciones:
Juárez-Gabriel J.:
Maestría en Ciencias del Ambiente, Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Tuxpan de Rodriguez Cano, Veracruz, Mexico
Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
Alegría-Sánchez D.:
Facultad de Ciencias Biológicas y Agropecuarias región Peñuela, Universidad Veracruzana, Amatlán de los Reyes, Veracruz, Mexico
Yáñez-Aguirre D.:
Facultad de Ciencias Biológicas y Agropecuarias región Peñuela, Universidad Veracruzana, Amatlán de los Reyes, Veracruz, Mexico
Grostieta E.:
Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
Álvarez-Castillo L.:
Posgrado en Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Av. Ciudad Universitaria 3000, C.P. 04510, Coyoacán, Ciudad de México, Mexico
Torres-Castro M.:
Laboratorio de Zoonosis y Otras Enfermedades Transmitidas por Vector, Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Yucatán, Mérida, Mexico
Aréchiga-Ceballos N.:
Dirección de Diagnóstico y Referencia, Instituto de Diagnóstico y Referencia Epidemiológicos Dr. Manuel Martínez Báez, Mexico City, Mexico
Moo-Llanes D.A.:
Grupo de Arbovirosis y Zoonosis, Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, Chiapas, Tapachula, Mexico
Alves F.M.:
Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil
Pérez-Brígido C.D.:
Hospital Veterinario, Facultad de Ciencias Biológicas y Agropecuarias, región Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz, Mexico
Aguilar-Tipacamú G.:
CA. Ecología y Diversidad Faunística, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, Mexico
López González C.A.:
CA. Ecología y Diversidad Faunística, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, Mexico
Becker I.:
Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
Pech-Canché J.M.:
Laboratorio de Vertebrados Terrestres, Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz, Mexico
Colunga-Salas P.:
Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Xalapa de Enríquez, Veracruz, Mexico
Sánchez-Montes S.:
Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
Laboratorio de Diagnóstico, Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz, Mexico
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