Relationship between biofilm-forming microorganisms (BFM: Staphylococcus aureus and Pseudomonas aeruginosa) and DEFB1 gene variants on ß-defensin levels in periprosthetic joint infection (PJI)
Por:
Fernández-Torres, J, Zamudio-Cuevas, Y, Martinez-Flores, K, Franco-Cendejas, R
Publicada:
1 dic 2024
Resumen:
Background The purpose of this study was to investigate the relationship between biofilm-forming microorganisms (BFM) and DEFB1 gene variants on beta-defensin levels in patients with periprosthetic joint infection (PJI) of Mexican origin. Methods and results One hundred and five clinical aspirates were obtained from patients with suspected PJI. After microbiologic culture, samples were classified as non-septic and septic; of the latter, only those positive for Staphylococcus aureus and Pseudomonas aeruginosa were selected. beta-Defensin levels were quantified by ELISA, DNA was extracted from total leukocytes of the samples, and - 20G > A (rs11362) and - 44 C > G (rs1800972) variants were genotyped using TaqMan probes. Forty-one clinical aspirates were non-septic, 18 were positive for S. aureus and 18 were positive for P. aeruginosa. It was observed that beta-defensin levels were higher in the P. aeruginosa group compared to S. aureus group (2339.0 pg/mL IQR = 1809.2 vs. 1821.3 pg/mL IQR = 1536.4) and non-septic group (2339.0 pg/mL IQR = 1809.2 vs. 1099.7 pg/mL IQR = 1744.5, P < 0.001). The CG genotype of the rs1800972 variant was associated with higher beta-defensin levels compared to the CC genotype for both P. aeruginosa and S. aureus (1905.8 vs. 421.7 pg/mL, P = 0.004; and 1878.2 vs. 256.4 pg/mL, P = 0.006, respectively). Conclusions Our results show that beta-defensin levels are significantly elevated in patients with BFM-associated PJI compared to those without infection. Furthermore, carriers of the CG genotype of the rs1800972 variant have an increased risk of PJI. Further research is needed to replicate these findings in a larger population.
Filiaciones:
Fernández-Torres, J:
Inst Nacl RehabilLuis Guillermo Ibarra Ibarra, Lab Liquido Sinovial, Mexico City 14389, Mexico
Univ Nacl Autonoma Mexico, Fac Quim, Dept Biol, Mexico City 04510, Mexico
Zamudio-Cuevas, Y:
Inst Nacl RehabilLuis Guillermo Ibarra Ibarra, Lab Liquido Sinovial, Mexico City 14389, Mexico
Martinez-Flores, K:
Inst Nacl RehabilLuis Guillermo Ibarra Ibarra, Lab Liquido Sinovial, Mexico City 14389, Mexico
Franco-Cendejas, R:
Inst Nacl RehabilLuis Guillermo Ibarra Ibarra, Div Infectol, Mexico City 14389, Mexico
Inst Nacl RehabilLuis Guillermo Ibarra Ibarra, Subdirecc Invest Biomed, Mexico City 14389, Mexico
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