Inhaled Litsea glaucescens K. (Lauraceae) leaves’ essential oil has anxiolytic and antidepressant-like activity in mice by BDNF pathway activation.
Por:
Díaz-Cantón J.K., Torres-Ramos M.A., Limón-Morales O., León-Santiago M., Rivero-Segura N.A., Tapia-Mendoza E., Guzmán-Gutiérrez S.L., Reyes-Chilpa R.
Publicada:
1 ene 2024
Ahead of Print:
1 dic 2023
Resumen:
Ethnopharmacological relevance: Litsea glaucescens K. (Lauraceae) is a small tree from the Mexican and Central American temperate forests, named as “Laurel”. Its aromatic leaves are ordinarily consumed as condiments, but also are important in Mexican Traditional Medicine, and among the most important non wood forest products in this area. The leaves are currently used in a decoction for the relief of sadness by the Mazahua ethnic group. Interestingly, “Laurel” has a long history. It was named as “Ehecapahtli” (wind medicine) in pre-Columbian times and applied to heal maladies correlated to the Central Nervous System, among them depression, according to botanical texts written in the American Continent almost five centuries ago. Aim of the study: Depression is the first cause of incapacity in the world, and society demands alternative treatments, including aromatherapy. We have previously demonstrated the antidepressant-like activity of L. glaucescens leaves’ essential oil (LEO), as well as their monoterpenes linalool, and beta-pinene by intraperitoneal route in a mice behavioral model. Here we now examined if LEO and linalool exhibit this property and anxiolytic activity when administered to mice by inhalation. We also investigated if these effects occur by BDNF pathway activation in the brain. Materials and methods: The LEO was prepared by distillation with water steam and analyzed by gas chromatography-mass spectrometry (GC-MS). The monoterpenes linalool, eucalyptol and ß-pinene were identified and quantified. Antidepressant type properties were determined with the Forced Swim Test (FST) on mice previously exposed to LEO or linalool in an inhalation chamber. The spontaneous locomotor activity and the sedative effect were assessed with the Open Field Test (OFT), and the Exploratory Cylinder (EC), respectively. The anxiolytic properties were investigated with the Elevated Plus Maze Apparatus (EPM) and the Hole Board Test (HBT). All experiments were video documented. The mice were subjected to euthanasia, and the brain hippocampus and prefrontal cortex were dissected. Results: The L. glaucescens essential oil (LEO) contains 31 compounds according to GC/MS, including eucalyptol, linalool and beta-pinene. The LEO has anxiolytic effect by inhalation in mice, as well as linalool, and ß-pinene, as indicated by OFT and EC tests. The LEO and imipramine have antidepressant like activity in mice as revealed by the FST; however, linalool and ketamine treatments didn't modify the time of immobility. The BDNF was increased in FST in mice treated with LEO in both areas of the brain as revealed by Western blot; but did not decrease the level of corticosterone in plasma. The OFT indicated that LEO and imipramine didn't reduce the spontaneous motor activity, while linalool and ketamine caused a significant decrease. Conclusion: Here we report by the first time that L. glaucescens leaves essential oil has anxiolytic effect by inhalation in mice, as well as linalool, and ß-pinene. This oil also maintains its antidepressant-like activity by this administration way, similarly to the previously determined intraperitoneally. Since inhalation is a common administration route for humans, our results suggest L. glaucescens essential oil deserve future investigation due to its potential application in aromatherapy. © 2023 Elsevier B.V.
Filiaciones:
Díaz-Cantón J.K.:
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Circuito Exterior S/N, Coyoacán, C.P. 04510, Ciudad de México, Mexico
Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico
Torres-Ramos M.A.:
Dirección de Investigación, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Av. Insurgentes Sur 3877, La Fama, Tlalpan, Ciudad de México, 14269, Mexico
Limón-Morales O.:
Departamento de Biología de la Reproducción, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Leyes de Reforma 1ra Secc, Iztapalapa, Ciudad de México, 09340, Mexico
León-Santiago M.:
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Circuito Exterior S/N, Coyoacán, C.P. 04510, Ciudad de México, Mexico
Rivero-Segura N.A.:
Dirección de Investigación, Instituto Nacional de Geriatría (INGER), Blvd. Adolfo Ruiz Cortines 2767, Mexico City, 10200, Mexico
Tapia-Mendoza E.:
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Circuito Exterior S/N, Coyoacán, C.P. 04510, Ciudad de México, Mexico
Guzmán-Gutiérrez S.L.:
CONAHCyT-Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Av. Universidad 3000, Circuito Escolar S/N, Coyoacán, C.P. 04510, Ciudad Universitaria, Ciudad de México, Mexico
Reyes-Chilpa R.:
Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Circuito Exterior S/N, Coyoacán, C.P. 04510, Ciudad de México, Mexico
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