Fragmentation of protonated oligonucleotides by energetic photons and Cq+ ions
Por:
González-Magaña O., Tiemens M., Reitsma G., Boschman L., Door M., Bari S., Lahaie P.O., Wagner J.R., Huels M.A., Hoekstra R., Schlathölter T.
Publicada:
1 ene 2013
Categoría:
Atomic and Molecular Physics, and Optics
Resumen:
The ionization and fragmentation of trapped protonated dGCAT oligonucleotides upon interaction with energetic photons (h?= 10-570 eV) and keV Cq+ ions was investigated by means of time-of-flight mass spectrometry. The observed fragmentation patterns are dominated by protonated and nonprotonated nucleobase ions and fragments of the deoxyribose moiety. Fragments exceeding the size of nucleosides are almost completely absent. Absorption of VUV photons as well as interaction with keV ions predominantly involves ionization or excitation of molecular valence electrons and accordingly the observed fragmentation patterns exhibit qualitatively similar features. Soft-x-ray-induced ionization of core level electrons accompanied by subsequent emission of an Auger electron shifts the fragment distributions towards smaller masses. This systematic study allows for insights into differences and similarities between ion- and photon-induced excitation and fragmentation mechanisms. In particular, the crucial role of the deoxyribose moiety for radiation-induced DNA damage that was predicted on the basis of gas-phase experiments using isolated deoxyribose molecules is confirmed. © 2013 American Physical Society.
Filiaciones:
González-Magaña O.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Tiemens M.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Reitsma G.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Boschman L.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Door M.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Bari S.:
European XFEL GmbH, Notkestrasse 85, 22607 Hamburg, Germany
Lahaie P.O.:
Department of Nuclear Medicine and Radiobiology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Wagner J.R.:
Department of Nuclear Medicine and Radiobiology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Huels M.A.:
Department of Nuclear Medicine and Radiobiology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC J1H 5N4, Canada
Hoekstra R.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
Schlathölter T.:
KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, 9747AA Groningen, Netherlands
|