Acetyl Phosphate Acetylates Proteins of Streptomyces coelicolor M-145
Por:
Takahashi-Iniguez, T., Flores, M. E.
Publicada:
1 ene 2023
Resumen:
Abstract: Protein Ne-lysine acetylation is a ubiquitous posttranslational modification. Acetylation in eukaryotes and prokaryotes can perform by acetylases or nonenzymatically using acetyl phosphate. Proteins involved in the carbon metabolism of Streptomyces coelicolor M-145 have been determined to be acetylated. Here, acetylation by acetyl phosphate of purified recombinant isocitrate dehydrogenase, malate dehydrogenase, phosphoenolpyruvate carboxylase, and phosphoenolpyruvate carboxykinase from S. coelicolor is reported. The enzymatic activities of isocitrate and malate dehydrogenases were negatively affected by acetylation, suggesting that acetyl phosphate could act as a metabolic regulator of glucose assimilation in this microorganism. To our knowledge, this is the first time that protein acetylation by acetyl phosphate has been reported in a streptomycete. © 2023, Pleiades Publishing, Inc.
Filiaciones:
Takahashi-Iniguez, T.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de Mexico, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Mexico City 04510, Mexico
Flores, M. E.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de Mexico, 04510, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Mol & Biotecnol, Mexico City 04510, Mexico
|